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Rigshospitalet - en del af Københavns Universitetshospital
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Analysis of HOXA9 methylated ctDNA in ovarian cancer using sense-antisense measurement

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  • Louise Faaborg
  • Rikke Fredslund Andersen
  • Marianne Waldstrøm
  • Estrid Høgdall
  • Claus Høgdall
  • Parvin Adimi
  • Anders Jakobsen
  • Karina Dahl Steffensen
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DNA promoter methylation is an early event in tumorigenesis and holds promise as a valuable marker in ovarian cancer (OC). It can be measured using circulating tumor specific DNA (ctDNA) isolated from the bloodstream. Sensitivity, however, is a limiting factor of its diagnostic feasibility in OC. DNA methylation analyses are based on bisulfite conversion, resulting in two DNA strands that are no longer complementary. The current standard strategy would then target only one of the double stranded DNA strands, but the potential to increase the sensitivity by targeting both DNA strands is available. In this study, we aimed at evaluating the diagnostic potential of methylated HOXA9 ctDNA in OC by targeting both the DNA sense and antisense strand. Methylated HOXA9 was detected in the plasma of 47/79 (59.5%) patients with newly diagnosed OC using sense-antisense droplet digital PCR. Simultaneous sense-antisense measurement increased the sensitivity by 14.6% (51.9% to 59.5%) as compared to antisense only. In patients with FIGO stage I-II disease the sensitivity was increased by 25%. In conclusion, simultaneous measurement targeting both DNA strands can increase the sensitivity and the analytical approach appears valuable in the diagnostic setting of OC.

OriginalsprogEngelsk
TidsskriftClinica chimica acta; international journal of clinical chemistry
Vol/bind522
Sider (fra-til)152-157
Antal sider6
ISSN0009-8981
DOI
StatusUdgivet - nov. 2021

Bibliografisk note

Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

ID: 70625405