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An exploratory study of microbial diversity in sinus infections of cystic fibrosis patients by molecular methods

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@article{830b5f1d792f49bf86bfb3dfe1046b7d,
title = "An exploratory study of microbial diversity in sinus infections of cystic fibrosis patients by molecular methods",
abstract = "BACKGROUND: For the first time microorganisms in CF sinuses are investigated by molecular methods in response to an absence of anaerobes in CF sinus samples during a two-year period at the Copenhagen CF center.METHODS: Endoscopic sinus surgery was performed in 19 CF patients. DNA from intact bacterial cells was investigated by 16S rRNA gene analysis and quantitative PCR. Results were compared to culture-dependent routine diagnosis.RESULTS: Molecular methods showed a large microbial diversity, which included undetected anaerobes that may play a pathogenic role. Importantly, the culture methods did not always detect known CF pathogens. Quantitative PCR showed generally a higher abundance of classic CF pathogens e.g. Pseudomonas aeruginosa and Staphylococcus aureus compared with the anaerobe Propionibacterium acnes.CONCLUSIONS: The results indicate that the culture methods in some cases may not be suitable as stand-alone method for this patient group, as diversity may be underestimated and important species undetected.",
author = "Rudkj{\o}bing, {Vibeke B{\o}rsholt} and Kasper Aanaes and Wolff, {Tine Yding} and {von Buchwald}, Christian and Johansen, {Helle Krogh} and Thomsen, {Trine Rolighed}",
note = "Copyright {\circledC} 2014 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.",
year = "2014",
month = "12",
doi = "10.1016/j.jcf.2014.02.008",
language = "English",
volume = "13",
pages = "645--52",
journal = "Journal of Cystic Fibrosis",
issn = "1569-1993",
publisher = "Elsevier BV",
number = "6",

}

RIS

TY - JOUR

T1 - An exploratory study of microbial diversity in sinus infections of cystic fibrosis patients by molecular methods

AU - Rudkjøbing, Vibeke Børsholt

AU - Aanaes, Kasper

AU - Wolff, Tine Yding

AU - von Buchwald, Christian

AU - Johansen, Helle Krogh

AU - Thomsen, Trine Rolighed

N1 - Copyright © 2014 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

PY - 2014/12

Y1 - 2014/12

N2 - BACKGROUND: For the first time microorganisms in CF sinuses are investigated by molecular methods in response to an absence of anaerobes in CF sinus samples during a two-year period at the Copenhagen CF center.METHODS: Endoscopic sinus surgery was performed in 19 CF patients. DNA from intact bacterial cells was investigated by 16S rRNA gene analysis and quantitative PCR. Results were compared to culture-dependent routine diagnosis.RESULTS: Molecular methods showed a large microbial diversity, which included undetected anaerobes that may play a pathogenic role. Importantly, the culture methods did not always detect known CF pathogens. Quantitative PCR showed generally a higher abundance of classic CF pathogens e.g. Pseudomonas aeruginosa and Staphylococcus aureus compared with the anaerobe Propionibacterium acnes.CONCLUSIONS: The results indicate that the culture methods in some cases may not be suitable as stand-alone method for this patient group, as diversity may be underestimated and important species undetected.

AB - BACKGROUND: For the first time microorganisms in CF sinuses are investigated by molecular methods in response to an absence of anaerobes in CF sinus samples during a two-year period at the Copenhagen CF center.METHODS: Endoscopic sinus surgery was performed in 19 CF patients. DNA from intact bacterial cells was investigated by 16S rRNA gene analysis and quantitative PCR. Results were compared to culture-dependent routine diagnosis.RESULTS: Molecular methods showed a large microbial diversity, which included undetected anaerobes that may play a pathogenic role. Importantly, the culture methods did not always detect known CF pathogens. Quantitative PCR showed generally a higher abundance of classic CF pathogens e.g. Pseudomonas aeruginosa and Staphylococcus aureus compared with the anaerobe Propionibacterium acnes.CONCLUSIONS: The results indicate that the culture methods in some cases may not be suitable as stand-alone method for this patient group, as diversity may be underestimated and important species undetected.

U2 - 10.1016/j.jcf.2014.02.008

DO - 10.1016/j.jcf.2014.02.008

M3 - Journal article

VL - 13

SP - 645

EP - 652

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

SN - 1569-1993

IS - 6

ER -

ID: 44955184