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Udgivet

A simple protocol for preparation of a liposomal vesicle with encapsulated plasmid DNA that mediate high accumulation and reporter gene activity in tumor tissue

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Cell-free DNA in newly diagnosed patients with glioblastoma - a clinical prospective feasibility study

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  2. Identification of Tumor Antigens Among the HLA Peptidomes of Glioblastoma Tumors and Plasma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Withdrawal: Identification of tumor antigens among the HLA peptidomes of glioblastoma tumors and plasma

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  4. Actively personalized vaccination trial for newly diagnosed glioblastoma

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  5. Extracranial metastases in glioblastoma-Two case stories

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The systemic delivery of gene therapeutics by non-viral methods has proven difficult. Transfection systems that are performing well in vitro have been reported to have disadvantageous properties such as rapid clearance and short circulation time often resulting in poor transfection efficiency when applied in vivo. Large unilaminary vesicles (LUV) with encapsulated nucleic acids designated stabilizedplasmid-lipo-particle (SPLP) have showed promising results in terms of systemic stability and accumulation in tumor tissue due to the enhanced permeability and retention effect (EPR). We have developed a simple protocol for the research-scale preparation of SPLPs from commercially available reagents with high amounts of encapsulated plasmid DNA. The SPLPs show properties of promising accumulation in tumor tissue in comparison to other organs when intravenously injected into xenograft tumor-bearing nude mice. Although transcriptionally targeted suicide gene therapy was not achieved, the SPLPs were capable of mediating reporter gene transfection in subcutaneous flank tumors originating from human small cell lung cancer.
OriginalsprogEngelsk
TidsskriftResults in Pharma Sciences
Vol/bind1
Udgave nummer1
Sider (fra-til)49-56
Antal sider8
ISSN2211-2863
DOI
StatusUdgivet - 3 sep. 2011

ID: 33142962