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Rigshospitalet - en del af Københavns Universitetshospital
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A novel double staining strategy for improved detection of testicular carcinoma in situ cells in human semen samples

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  1. Effect of bipolar radiofrequency thermotherapy on benign prostate hyperplasia

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Anogenital distance and reproductive parameters in young men

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. Prolactin and pituitary-gonadal function in cigarette smoking infertile patients

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  1. Activin A determines steroid levels and composition in the fetal testis

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  2. Distinguishing between hidden testes and anorchia: The role of endocrine evaluation in infancy and childhood

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

  3. High serum FSH is not a risk factor for low bone mineral density in infertile men

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Vis graf over relationer
Prompted by the recently reported expression of POU5F1 (OCT3/4) in epididymis, a panel of markers for carcinoma in situ (CIS) testis and testicular germ cell tumours (TGCT), including AP-2γ(TFAP2C), NANOG, OCT3/4, KIT, placental-like alkaline phosphatase (PLAP), M2A/PDPN and MAGE-A4 were examined by immunohistochemistry or in situ hybridisation in urogenital epithelia, which may interfere with detection of CIS cells in semen. In addition to OCT3/4, the expression of AP-2γ and NANOG or their variants was detected in urogenital epithelia, while other CIS markers, including PLAP/alkaline phosphatase were absent. A combination of immunocytological staining for AP-2γ or OCT3/4 and rapid cytochemical alkaline phosphatase reaction was subsequently developed. This approach was tested in 22 patients with TGCT. In 14 patients (63.6%), double stained cells were found and thus the method was proven suitable for the detection of CIS cells in semen. In conclusion, transcription factors related to pluripotency and undifferentiated state of cells, which most likely have several variants or modifications, are unexpectedly detected using currently available antibodies in urogenital epithelial cells which may be shed into semen. Combining the immunohistochemical nuclear markers with a rapid cytochemical alkaline phosphatase reaction for detection of CIS cells in ejaculates may provide a more reliable diagnostic method.
OriginalsprogEngelsk
TidsskriftAndrologia
Sider (fra-til)78-85
Antal sider8
ISSN0303-4569
DOI
StatusUdgivet - 2012

ID: 32785190