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Hvidovre Hospital - a part of Copenhagen University Hospital
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A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

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  • Mohsen Mohammadniaei
  • Ming Zhang
  • Jon Ashley
  • Ulf Bech Christensen
  • Lennart Jan Friis-Hansen
  • Rasmus Gregersen
  • Jan Gorm Lisby
  • Thomas Lars Benfield
  • Finn Erland Nielsen
  • Jens Henning Rasmussen
  • Ellen Bøtker Pedersen
  • Anne Christine Rye Olinger
  • Lærke Tørring Kolding
  • Maryam Naseri
  • Tao Zheng
  • Wentao Wang
  • Jan Gorodkin
  • Yi Sun
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The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL-1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.

Original languageEnglish
Article number5089
JournalNature Communications
Volume12
Issue number1
Pages (from-to)1-12
Number of pages12
DOIs
Publication statusPublished - 24 Aug 2021

    Research areas

  • COVID-19 Nucleic Acid Testing/methods, COVID-19 Testing, COVID-19/diagnosis, Humans, Nucleic Acid Amplification Techniques/methods, RNA, Viral/genetics, Recombination, Genetic, SARS-CoV-2/genetics

ID: 67304414