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Hvidovre Hospital - en del af Københavns Universitetshospital

Characterization of Third-Generation Cephalosporin-Resistant Escherichia coli from Bloodstream Infections in Denmark

Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review


  1. Nasal carriage of methicillin resistant staphylococci

    Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review

  • Frank Hansen
  • Stefan S Olsen
  • Ole Heltberg
  • Ulrik S Justesen
  • David Fuglsang-Damgaard
  • Inge Jenny Dahl Knudsen
  • Anette M Hammerum
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The aim of the study was to investigate the molecular epidemiology of 87 third-generation cephalosporin-resistant Escherichia coli (3GC-R Ec) from bloodstream infections in Denmark from 2009. Sixty-eight of the 87 isolates were extended-spectrum beta-lactamase (ESBL) producers, whereas 17 isolates featured AmpC mutations only (without a coexpressed ESBL enzyme) and 2 isolates were producing CMY-22. The majority (82%) of the ESBL-producing isolates in our study were CTX-M-15 producers and primarily belonged to phylogroup B2 (54.4%) or D (23.5%). Further, one of the two CMY-22-producing isolates belonged to B2, whereas only few of the other AmpCs isolates belonged to B2 and D. Pulsed-field gel electrophoresis revealed that both clonal and nonclonal spread of 3GC-R Ec occurred. ST131 was detected in 50% of ESBL-producing isolates. The remaining ESBL-producing isolates belonged to 17 other sequence types (STs), including several other internationally spreading STs (e.g., ST10, ST69, and ST405). The majority (93%) of the ESBL-producing isolates and one of the CMY-22-producing isolates were multiresistant. In conclusion, 3GC-R in bacteriaemic E. coli in Denmark was mostly due to ESBL production, overexpression of AmpC, and to a lesser extent to plasmid-mediated AmpC. The worldwide disseminated CTX-M-15-ST131 was strongly represented in this collection of Danish, bacteriaemic E. coli isolates.

TidsskriftMicrobial drug resistance (Larchmont, N.Y.)
Udgave nummer4
Sider (fra-til)316-324
StatusUdgivet - 2014

ID: 44109360