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A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

Publikation: Bidrag til tidsskriftTidsskriftartikelpeer review

Harvard

Mohammadniaei, M, Zhang, M, Ashley, J, Christensen, UB, Friis-Hansen, LJ, Gregersen, R, Lisby, JG, Benfield, TL, Nielsen, FE, Henning Rasmussen, J, Pedersen, EB, Olinger, ACR, Kolding, LT, Naseri, M, Zheng, T, Wang, W, Gorodkin, J & Sun, Y 2021, 'A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA', Nature Communications, bind 12, nr. 1, 5089, s. 1-12. https://doi.org/10.1038/s41467-021-25387-9

APA

Mohammadniaei, M., Zhang, M., Ashley, J., Christensen, U. B., Friis-Hansen, L. J., Gregersen, R., Lisby, J. G., Benfield, T. L., Nielsen, F. E., Henning Rasmussen, J., Pedersen, E. B., Olinger, A. C. R., Kolding, L. T., Naseri, M., Zheng, T., Wang, W., Gorodkin, J., & Sun, Y. (2021). A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. Nature Communications, 12(1), 1-12. [5089]. https://doi.org/10.1038/s41467-021-25387-9

CBE

Mohammadniaei M, Zhang M, Ashley J, Christensen UB, Friis-Hansen LJ, Gregersen R, Lisby JG, Benfield TL, Nielsen FE, Henning Rasmussen J, Pedersen EB, Olinger ACR, Kolding LT, Naseri M, Zheng T, Wang W, Gorodkin J, Sun Y. 2021. A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. Nature Communications. 12(1):1-12. https://doi.org/10.1038/s41467-021-25387-9

MLA

Vancouver

Mohammadniaei M, Zhang M, Ashley J, Christensen UB, Friis-Hansen LJ, Gregersen R o.a. A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. Nature Communications. 2021 aug 24;12(1):1-12. 5089. https://doi.org/10.1038/s41467-021-25387-9

Author

Mohammadniaei, Mohsen ; Zhang, Ming ; Ashley, Jon ; Christensen, Ulf Bech ; Friis-Hansen, Lennart Jan ; Gregersen, Rasmus ; Lisby, Jan Gorm ; Benfield, Thomas Lars ; Nielsen, Finn Erland ; Henning Rasmussen, Jens ; Pedersen, Ellen Bøtker ; Olinger, Anne Christine Rye ; Kolding, Lærke Tørring ; Naseri, Maryam ; Zheng, Tao ; Wang, Wentao ; Gorodkin, Jan ; Sun, Yi. / A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA. I: Nature Communications. 2021 ; Bind 12, Nr. 1. s. 1-12.

Bibtex

@article{23000c39b1454afc9d2e28a192204c8c,
title = "A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA",
abstract = "The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL-1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.",
keywords = "COVID-19 Nucleic Acid Testing/methods, COVID-19 Testing, COVID-19/diagnosis, Humans, Nucleic Acid Amplification Techniques/methods, RNA, Viral/genetics, Recombination, Genetic, SARS-CoV-2/genetics",
author = "Mohsen Mohammadniaei and Ming Zhang and Jon Ashley and Christensen, {Ulf Bech} and Friis-Hansen, {Lennart Jan} and Rasmus Gregersen and Lisby, {Jan Gorm} and Benfield, {Thomas Lars} and Nielsen, {Finn Erland} and {Henning Rasmussen}, Jens and Pedersen, {Ellen B{\o}tker} and Olinger, {Anne Christine Rye} and Kolding, {L{\ae}rke T{\o}rring} and Maryam Naseri and Tao Zheng and Wentao Wang and Jan Gorodkin and Yi Sun",
note = "{\textcopyright} 2021. The Author(s).",
year = "2021",
month = aug,
day = "24",
doi = "10.1038/s41467-021-25387-9",
language = "English",
volume = "12",
pages = "1--12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

AU - Mohammadniaei, Mohsen

AU - Zhang, Ming

AU - Ashley, Jon

AU - Christensen, Ulf Bech

AU - Friis-Hansen, Lennart Jan

AU - Gregersen, Rasmus

AU - Lisby, Jan Gorm

AU - Benfield, Thomas Lars

AU - Nielsen, Finn Erland

AU - Henning Rasmussen, Jens

AU - Pedersen, Ellen Bøtker

AU - Olinger, Anne Christine Rye

AU - Kolding, Lærke Tørring

AU - Naseri, Maryam

AU - Zheng, Tao

AU - Wang, Wentao

AU - Gorodkin, Jan

AU - Sun, Yi

N1 - © 2021. The Author(s).

PY - 2021/8/24

Y1 - 2021/8/24

N2 - The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL-1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.

AB - The current nucleic acid signal amplification methods for SARS-CoV-2 RNA detection heavily rely on the functions of biological enzymes which imposes stringent transportation and storage conditions, high cost and global supply shortages. Here, a non-enzymatic whole genome detection method based on a simple isothermal signal amplification approach is developed for rapid detection of SARS-CoV-2 RNA and potentially any types of nucleic acids regardless of their size. The assay, termed non-enzymatic isothermal strand displacement and amplification (NISDA), is able to quantify 10 RNA copies.µL-1. In 164 clinical oropharyngeal RNA samples, NISDA assay is 100 % specific, and it is 96.77% and 100% sensitive when setting up in the laboratory and hospital, respectively. The NISDA assay does not require RNA reverse-transcription step and is fast (<30 min), affordable, highly robust at room temperature (>1 month), isothermal (42 °C) and user-friendly, making it an excellent assay for broad-based testing.

KW - COVID-19 Nucleic Acid Testing/methods

KW - COVID-19 Testing

KW - COVID-19/diagnosis

KW - Humans

KW - Nucleic Acid Amplification Techniques/methods

KW - RNA, Viral/genetics

KW - Recombination, Genetic

KW - SARS-CoV-2/genetics

UR - http://www.scopus.com/inward/record.url?scp=85113296116&partnerID=8YFLogxK

U2 - 10.1038/s41467-021-25387-9

DO - 10.1038/s41467-021-25387-9

M3 - Journal article

C2 - 34429424

VL - 12

SP - 1

EP - 12

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 5089

ER -

ID: 67304414