Whole genome sequencing reveals two genetically distinct MRSA outbreaks among people who inject drugs and homeless people in Copenhagen

Mette Damkjaer Bartels, Mona Katrine Alberthe Holm, Peder Worning, Bodil Stavad, Uffe Vest Schneider, Heidi Meiniche, Henrik Thiesen, Henrik Westh

1 Citation (Scopus)

Abstract

Whole genome sequencing (WGS) has greatly improved the detection of methicillin-resistant Staphylococcus aureus (MRSA) transmission between people. We describe the transmission of two unique MRSA clones among homeless people in Copenhagen using WGS and core genome MLST (cgMLST). In 2014, an accumulation of MRSA bacteremia cases among homeless people admitted to our hospital was recognized, all having the rare MRSA spa t5147/ST88. The European Typology of Homelessness and Housing Exclusion (ETHOS) categories revealed that people who inject drugs (PWID) frequently visiting the milieu but living in private accommodation accounted for most cases. Hoping to terminate the transmission, 161 homeless people were MRSA screened in 2015, but no additional cases were found. From 2009 to 2018, 60 patients with genomically related t5147/ST88 isolates were found, of these 70% were confirmed to come from the homeless setting and 17% had bacteremia. From 2017 to 2020, cgMLST revealed a smaller MRSA outbreak including 13 PWID with a completely different clone, t1476/ST8, of which 15% had bacteremia. Our study confirms that WGS and cgMLST is excellent to reveal MRSA outbreaks. The ETHOS categorization can be useful to find the primary source of spread in the homeless community.

Original languageEnglish
JournalAPMIS - Journal of Pathology, Microbiology and Immunology
Volume131
Issue number6
Pages (from-to)294-302
Number of pages9
ISSN0903-4641
DOIs
Publication statusPublished - Jun 2023

Keywords

  • Bacteremia/epidemiology
  • Disease Outbreaks
  • Drug Users
  • Humans
  • Ill-Housed Persons
  • Methicillin-Resistant Staphylococcus aureus/genetics
  • Multilocus Sequence Typing
  • Staphylococcal Infections
  • Substance Abuse, Intravenous
  • Whole Genome Sequencing

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