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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Von Frey testing revisited - provision of an online algorithm for improved accuracy of 50% thresholds

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BACKGROUND: In the pain field, it is essential to quantify nociceptive responses. The response to the application of von Frey filaments to the skin measures tactile sensitivity and is a surrogate marker of allodynia in states of peripheral and/or central sensitization. The method is widely used across species within the pain field. However, uncertainties appear to exist regarding the appropriate method for analysing obtained data. Therefore, there is a need for refinement of the calculations for transformation of raw data to quantifiable data.

METHODS: Here, we briefly review the fundamentals behind von Frey testing using the standard up-down method and the associated statistics and show how different parameters of the statistical equation influence the calculated 50% threshold results. We discuss how to obtain the most accurate estimations in a given experimental setting.

RESULTS: To enhance accuracy and reproducibility across laboratories, we present an easy to use algorithm that calculates 50% thresholds based on the exact filaments and their interval using math beyond the traditional methods. This tool is available to the everyday user of von Frey filaments and allows the insertion of all imaginable ranges of filaments and is thus applicable to data derived in any species.

CONCLUSION: We advocate for the use of this algorithm in order to minimise inaccuracies and to improve internal and external reproducibility.

Original languageEnglish
JournalEuropean journal of pain (London, England)
Pages (from-to)epub
ISSN1090-3801
DOIs
Publication statusPublished - 30 Dec 2019

ID: 58917316