Volume-referent bone turnover estimated from the interlabel area fraction after sequential labeling

Thomas Frisch*, M. S. Sørensen, S. Overgaard, M. Lind, P. Bretlau

*Corresponding author for this work
37 Citations (Scopus)

Abstract

In the compact bony otic capsule remodeling is low, and bone remodeling units are distributed centrifugally in relation to inner ear tissues and spaces. Fluorochrome-labeled bone remodeling units are scarce, abortive, and tortuous with no uniform direction of movement. This study presents a method for the estimation of volume-referent bone turnover based on measurements of the fractional area between labels after sequential labeling with osteofluorochromes. The applicability of this method is tested against a classical quantification method in undecalcified cortical specimens from the canine humerus, where both methods can be used. The estimate of bone turnover derived from the new sequential labeling in eight dogs is 7.4% (SEM 2.1%) per year and the classic estimate derived from calculations of the formative osteonal area and the formative period yields 6.9% (SEM 2.1%) per year. Agreement is sufficient to justify future measurements of absolute bone turnover in sequentially labeled perilabyrinthine bone.

Original languageEnglish
JournalBone
Volume22
Issue number6
Pages (from-to)677-682
Number of pages6
ISSN8756-3282
DOIs
Publication statusPublished - Jun 1998

Keywords

  • Bone remodeling
  • Bone turnover
  • Calcein
  • Cortical bone
  • Histomorphometry
  • Tetracycline

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