Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract

Martin Blomberg Jensen; John E Nielsen; Anne Jørgensen; Ewa Rajpert-De Meyts; David Møbjerg Kristensen; Niels Jørgensen; Niels Erik Skakkebæk; Anders Juul; Henrik Leffers

doi:10.1093/humrep/deq024

Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract

Martin Blomberg Jensen, John E Nielsen, Anne Jørgensen, Ewa Rajpert-De Meyts, David Møbjerg Kristensen, Niels Jørgensen, Niels Erik Skakkebæk, Anders Juul, Henrik Leffers

Department of Growth and Reproduction

278 Citations (Scopus)

Abstract

The vitamin D receptor (VDR) is expressed in human testis, and vitamin D (VD) has been suggested to affect survival and function of mature spermatozoa. Indeed, VDR knockout mice and VD deficient rats show decreased sperm counts and low fertility. However, the cellular response to VD is complex, since it is not solely dependent on VDR expression, but also on cellular uptake of circulating VD and presence and activity of VD metabolizing enzymes. Expression of VD metabolizing enzymes has not previously been investigated in human testis and male reproductive tract. Therefore, we performed a comprehensive analysis of the expression of VDR, VD activating (CYP2R1, CYP27A1, CYP27B1) and inactivating (CYP24A1) enzymes in the testis, epididymis, seminal vesicle (SV), prostate and spermatozoa.

Original language	English
Journal	Human Reproduction
Volume	25
Issue number	5
Pages (from-to)	1303-11
Number of pages	9
ISSN	0268-1161
DOIs	https://doi.org/10.1093/humrep/deq024
Publication status	Published - 1 May 2010

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title = "Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract",

abstract = "The vitamin D receptor (VDR) is expressed in human testis, and vitamin D (VD) has been suggested to affect survival and function of mature spermatozoa. Indeed, VDR knockout mice and VD deficient rats show decreased sperm counts and low fertility. However, the cellular response to VD is complex, since it is not solely dependent on VDR expression, but also on cellular uptake of circulating VD and presence and activity of VD metabolizing enzymes. Expression of VD metabolizing enzymes has not previously been investigated in human testis and male reproductive tract. Therefore, we performed a comprehensive analysis of the expression of VDR, VD activating (CYP2R1, CYP27A1, CYP27B1) and inactivating (CYP24A1) enzymes in the testis, epididymis, seminal vesicle (SV), prostate and spermatozoa.",

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year = "2010",

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doi = "10.1093/humrep/deq024",

language = "English",

volume = "25",

pages = "1303--11",

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T1 - Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract

AU - Blomberg Jensen, Martin

AU - Nielsen, John E

AU - Jørgensen, Anne

AU - Rajpert-De Meyts, Ewa

AU - Kristensen, David Møbjerg

AU - Jørgensen, Niels

AU - Skakkebæk, Niels Erik

AU - Juul, Anders

AU - Leffers, Henrik

PY - 2010/5/1

Y1 - 2010/5/1

N2 - The vitamin D receptor (VDR) is expressed in human testis, and vitamin D (VD) has been suggested to affect survival and function of mature spermatozoa. Indeed, VDR knockout mice and VD deficient rats show decreased sperm counts and low fertility. However, the cellular response to VD is complex, since it is not solely dependent on VDR expression, but also on cellular uptake of circulating VD and presence and activity of VD metabolizing enzymes. Expression of VD metabolizing enzymes has not previously been investigated in human testis and male reproductive tract. Therefore, we performed a comprehensive analysis of the expression of VDR, VD activating (CYP2R1, CYP27A1, CYP27B1) and inactivating (CYP24A1) enzymes in the testis, epididymis, seminal vesicle (SV), prostate and spermatozoa.

AB - The vitamin D receptor (VDR) is expressed in human testis, and vitamin D (VD) has been suggested to affect survival and function of mature spermatozoa. Indeed, VDR knockout mice and VD deficient rats show decreased sperm counts and low fertility. However, the cellular response to VD is complex, since it is not solely dependent on VDR expression, but also on cellular uptake of circulating VD and presence and activity of VD metabolizing enzymes. Expression of VD metabolizing enzymes has not previously been investigated in human testis and male reproductive tract. Therefore, we performed a comprehensive analysis of the expression of VDR, VD activating (CYP2R1, CYP27A1, CYP27B1) and inactivating (CYP24A1) enzymes in the testis, epididymis, seminal vesicle (SV), prostate and spermatozoa.

U2 - 10.1093/humrep/deq024

DO - 10.1093/humrep/deq024

M3 - Journal article

C2 - 20172873

SN - 0268-1161

VL - 25

SP - 1303

EP - 1311

JO - Human Reproduction

JF - Human Reproduction

IS - 5

ER -

Vitamin D receptor and vitamin D metabolizing enzymes are expressed in the human male reproductive tract

Abstract

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