Vascular endothelin ET(B) receptor-mediated contraction requires phosphorylation of ERK1/2 proteins

Guogang Luo; Roya Jamali; Yong-Xiao Cao; Lars Edvinsson; Cang-Bao Xu

doi:10.1016/j.ejphar.2006.03.057

Vascular endothelin ET(B) receptor-mediated contraction requires phosphorylation of ERK1/2 proteins

Guogang Luo, Roya Jamali, Yong-Xiao Cao, Lars Edvinsson, Cang-Bao Xu

Department of Clinical Experimental Research

27 Citations (Scopus)

Abstract

In cardiovascular diseases, endothelin type B (ET(B)) receptors in arterial smooth muscle cells are upregulated. The present study revealed that organ culture of rat mesenteric artery segments enhanced endothelin ET(B) receptor-mediated contraction paralleled with increase in the receptor mRNA and protein expressions. The endothelin ET(B) receptor-mediated contraction was associated with increase in phosphorylation of extracellular regulation kinase 1 and 2 (ERK1/2) proteins and elevated levels of intracellular calcium. The elevation curve of intracellular calcium consisted of two phases: one rapid and one sustained. Inhibition of ERK1/2 phosphorylation by SB386023 or blockage of calcium channels by nifedipine significantly reduced the endothelin ET(B) receptor-mediated contraction (P<0.05) and decreased the sustained phase of intracellular calcium level, but not the rapid phase. Thus, phosphorylation of ERK1/2 proteins and elevation of intracellular calcium level are required for endothelin ET(B) receptor-mediated contraction in rat mesenteric artery.

Original language	English
Journal	European Journal of Pharmacology
Volume	538
Issue number	1-3
Pages (from-to)	124-31
Number of pages	8
ISSN	0014-2999
DOIs	https://doi.org/10.1016/j.ejphar.2006.03.057
Publication status	Published - 24 May 2006

Keywords

Animals
Blotting, Western
Calcium
Calcium Channel Blockers
Dose-Response Relationship, Drug
Enzyme Inhibitors
In Vitro Techniques
Intracellular Fluid
Mesenteric Arteries
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Nifedipine
Phosphorylation
RNA, Messenger
Rats
Rats, Sprague-Dawley
Receptor, Endothelin B
Time Factors
Up-Regulation
Vasoconstriction
Vasoconstrictor Agents
Viper Venoms

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10.1016/j.ejphar.2006.03.057

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title = "Vascular endothelin ET(B) receptor-mediated contraction requires phosphorylation of ERK1/2 proteins",

abstract = "In cardiovascular diseases, endothelin type B (ET(B)) receptors in arterial smooth muscle cells are upregulated. The present study revealed that organ culture of rat mesenteric artery segments enhanced endothelin ET(B) receptor-mediated contraction paralleled with increase in the receptor mRNA and protein expressions. The endothelin ET(B) receptor-mediated contraction was associated with increase in phosphorylation of extracellular regulation kinase 1 and 2 (ERK1/2) proteins and elevated levels of intracellular calcium. The elevation curve of intracellular calcium consisted of two phases: one rapid and one sustained. Inhibition of ERK1/2 phosphorylation by SB386023 or blockage of calcium channels by nifedipine significantly reduced the endothelin ET(B) receptor-mediated contraction (P<0.05) and decreased the sustained phase of intracellular calcium level, but not the rapid phase. Thus, phosphorylation of ERK1/2 proteins and elevation of intracellular calcium level are required for endothelin ET(B) receptor-mediated contraction in rat mesenteric artery.",

keywords = "Animals, Blotting, Western, Calcium, Calcium Channel Blockers, Dose-Response Relationship, Drug, Enzyme Inhibitors, In Vitro Techniques, Intracellular Fluid, Mesenteric Arteries, Mitogen-Activated Protein Kinase 1, Mitogen-Activated Protein Kinase 3, Nifedipine, Phosphorylation, RNA, Messenger, Rats, Rats, Sprague-Dawley, Receptor, Endothelin B, Time Factors, Up-Regulation, Vasoconstriction, Vasoconstrictor Agents, Viper Venoms",

author = "Guogang Luo and Roya Jamali and Yong-Xiao Cao and Lars Edvinsson and Cang-Bao Xu",

year = "2006",

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language = "English",

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T1 - Vascular endothelin ET(B) receptor-mediated contraction requires phosphorylation of ERK1/2 proteins

AU - Luo, Guogang

AU - Jamali, Roya

AU - Cao, Yong-Xiao

AU - Edvinsson, Lars

AU - Xu, Cang-Bao

PY - 2006/5/24

Y1 - 2006/5/24

N2 - In cardiovascular diseases, endothelin type B (ET(B)) receptors in arterial smooth muscle cells are upregulated. The present study revealed that organ culture of rat mesenteric artery segments enhanced endothelin ET(B) receptor-mediated contraction paralleled with increase in the receptor mRNA and protein expressions. The endothelin ET(B) receptor-mediated contraction was associated with increase in phosphorylation of extracellular regulation kinase 1 and 2 (ERK1/2) proteins and elevated levels of intracellular calcium. The elevation curve of intracellular calcium consisted of two phases: one rapid and one sustained. Inhibition of ERK1/2 phosphorylation by SB386023 or blockage of calcium channels by nifedipine significantly reduced the endothelin ET(B) receptor-mediated contraction (P<0.05) and decreased the sustained phase of intracellular calcium level, but not the rapid phase. Thus, phosphorylation of ERK1/2 proteins and elevation of intracellular calcium level are required for endothelin ET(B) receptor-mediated contraction in rat mesenteric artery.

AB - In cardiovascular diseases, endothelin type B (ET(B)) receptors in arterial smooth muscle cells are upregulated. The present study revealed that organ culture of rat mesenteric artery segments enhanced endothelin ET(B) receptor-mediated contraction paralleled with increase in the receptor mRNA and protein expressions. The endothelin ET(B) receptor-mediated contraction was associated with increase in phosphorylation of extracellular regulation kinase 1 and 2 (ERK1/2) proteins and elevated levels of intracellular calcium. The elevation curve of intracellular calcium consisted of two phases: one rapid and one sustained. Inhibition of ERK1/2 phosphorylation by SB386023 or blockage of calcium channels by nifedipine significantly reduced the endothelin ET(B) receptor-mediated contraction (P<0.05) and decreased the sustained phase of intracellular calcium level, but not the rapid phase. Thus, phosphorylation of ERK1/2 proteins and elevation of intracellular calcium level are required for endothelin ET(B) receptor-mediated contraction in rat mesenteric artery.

KW - Animals

KW - Blotting, Western

KW - Calcium

KW - Calcium Channel Blockers

KW - Dose-Response Relationship, Drug

KW - Enzyme Inhibitors

KW - In Vitro Techniques

KW - Intracellular Fluid

KW - Mesenteric Arteries

KW - Mitogen-Activated Protein Kinase 1

KW - Mitogen-Activated Protein Kinase 3

KW - Nifedipine

KW - Phosphorylation

KW - RNA, Messenger

KW - Rats

KW - Rats, Sprague-Dawley

KW - Receptor, Endothelin B

KW - Time Factors

KW - Up-Regulation

KW - Vasoconstriction

KW - Vasoconstrictor Agents

KW - Viper Venoms

UR - https://www.scopus.com/pages/publications/33646499656

U2 - 10.1016/j.ejphar.2006.03.057

DO - 10.1016/j.ejphar.2006.03.057

M3 - Journal article

C2 - 16650404

SN - 0014-2999

VL - 538

SP - 124

EP - 131

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 1-3

ER -

Vascular endothelin ET(B) receptor-mediated contraction requires phosphorylation of ERK1/2 proteins

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Keywords

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