Validation of RNAi by real time PCR

Knud Josefsen, Ying Chiu Lee

1 Citation (Scopus)

Abstract

Real time PCR is the analytic tool of choice for quantification of gene expression, while RNAi is concerned with downregulation of gene expression. Together, they constitute a powerful approach in any loss of function studies of selective genes. We illustrate here the use of real time PCR to verify luciferase mRNA silencing.
Original languageEnglish
JournalMethods in Molecular Biology
Volume703
Pages (from-to)205-17
Number of pages13
ISSN1064-3745
DOIs
Publication statusPublished - 2011

Keywords

  • DNA Primers
  • Gene Expression
  • HeLa Cells
  • Humans
  • Luciferases
  • Organic Chemicals
  • Polymerase Chain Reaction
  • RNA Interference

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