Validation of RNAi by real time PCR

Knud Josefsen, Ying Chiu Lee

1 Citation (Scopus)


Real time PCR is the analytic tool of choice for quantification of gene expression, while RNAi is concerned with downregulation of gene expression. Together, they constitute a powerful approach in any loss of function studies of selective genes. We illustrate here the use of real time PCR to verify luciferase mRNA silencing.
Original languageEnglish
JournalMethods in Molecular Biology
Pages (from-to)205-17
Number of pages13
Publication statusPublished - 2011


  • DNA Primers
  • Gene Expression
  • HeLa Cells
  • Humans
  • Luciferases
  • Organic Chemicals
  • Polymerase Chain Reaction
  • RNA Interference


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