Validation of MICA Legionella for Enumeration of Legionella pneumophila in sanitary waters and cooling tower waters: AOAC Performance Tested MethodSM #032201

Fanny Passot; Sabine Peslier; M Joseph Benzinger; Jonathan Blackburn; Wesley Thompson; Benjamin Bastin; Audrey Dumont; Sam Dukan

doi:10.1093/jaoacint/qsac150

Validation of MICA Legionella for Enumeration of Legionella pneumophila in sanitary waters and cooling tower waters: AOAC Performance Tested MethodSM #032201

Fanny Passot, Sabine Peslier, M Joseph Benzinger, Jonathan Blackburn, Wesley Thompson, Benjamin Bastin, Audrey Dumont, Sam Dukan

1 Citation (Scopus)

Abstract

BACKGROUND: Frequent testing for Legionella concentration in water is required by most health risk monitoring organizations worldwide. Domestic hot water and cooling tower water networks must be regularly controlled to prevent Legionnaires' disease, a potentially deadly lung infection. MICA Legionella is the fastest culture-based detection method for all serogroups of Legionella pneumophila, with automatic enumeration in 48 h and no need for confirmation.

OBJECTIVE: This study compares the performance and robustness of MICA Legionella with the reference method ISO 11731:2017 for the enumeration of culturable L. pneumophila.

METHODS: MICA Legionella and ISO 11731:2017 results were compared for domestic hot water and cooling tower water. Inclusivity and exclusivity were tested on reference and environmental strains. Ruggedness, lot-to-lot consistency, and stability of the reagents kit were also studied.

RESULTS: Enumeration of L. pneumophila by MICA Legionella was statistically equivalent to ISO 11731:2017 in both matrixes. In cooling tower waters, MICA Legionella showed better sensitivity than ISO 11731:2017. It presented a 94% sensitivity and a 97% specificity.

CONCLUSION: MICA Legionella is a highly sensitive and specific method for culturable L. pneumophila enumeration. It presents, in 48 hours, equivalent or better results than ISO 11731:2017. Its protocol is robust to variations. Its reagents kit is stable for up to 18 months.

HIGHLIGHTS: MICA Legionella is a robust and reliable method for the enumeration of culturable L. pneumophila in domestic and cooling tower water. It reduces significantly the number of sample pretreatments required in ISO 11731:2017. Automatic identification and enumeration of L. pneumophila microcolonies eliminates the requirement to have skilled analysts and limits the results variability. It also greatly reduces the time to results to 48 h instead of 7-10 days with ISO 11731:2017 while providing statistically equivalent results.

Original language	English
Journal	Journal of AOAC International
Volume	106
Issue number	3
Pages (from-to)	725-736
Number of pages	12
ISSN	1060-3271
DOIs	https://doi.org/10.1093/jaoacint/qsac150
Publication status	Published - 3 May 2023
Externally published	Yes

Keywords

Humans
Legionella
Legionella pneumophila
Legionnaires' Disease/prevention & control
Water
Water Microbiology

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Validation of MICA Legionella for Enumeration of Legionella pneumophila in sanitary waters and cooling tower waters: AOAC Performance Tested MethodSM #032201. / Passot, Fanny; Peslier, Sabine; Benzinger, M Joseph et al.
In: Journal of AOAC International, Vol. 106, No. 3, 03.05.2023, p. 725-736.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Validation of MICA Legionella for Enumeration of Legionella pneumophila in sanitary waters and cooling tower waters: AOAC Performance Tested MethodSM #032201",

abstract = "BACKGROUND: Frequent testing for Legionella concentration in water is required by most health risk monitoring organizations worldwide. Domestic hot water and cooling tower water networks must be regularly controlled to prevent Legionnaires' disease, a potentially deadly lung infection. MICA Legionella is the fastest culture-based detection method for all serogroups of Legionella pneumophila, with automatic enumeration in 48 h and no need for confirmation.OBJECTIVE: This study compares the performance and robustness of MICA Legionella with the reference method ISO 11731:2017 for the enumeration of culturable L. pneumophila.METHODS: MICA Legionella and ISO 11731:2017 results were compared for domestic hot water and cooling tower water. Inclusivity and exclusivity were tested on reference and environmental strains. Ruggedness, lot-to-lot consistency, and stability of the reagents kit were also studied.RESULTS: Enumeration of L. pneumophila by MICA Legionella was statistically equivalent to ISO 11731:2017 in both matrixes. In cooling tower waters, MICA Legionella showed better sensitivity than ISO 11731:2017. It presented a 94% sensitivity and a 97% specificity.CONCLUSION: MICA Legionella is a highly sensitive and specific method for culturable L. pneumophila enumeration. It presents, in 48 hours, equivalent or better results than ISO 11731:2017. Its protocol is robust to variations. Its reagents kit is stable for up to 18 months.HIGHLIGHTS: MICA Legionella is a robust and reliable method for the enumeration of culturable L. pneumophila in domestic and cooling tower water. It reduces significantly the number of sample pretreatments required in ISO 11731:2017. Automatic identification and enumeration of L. pneumophila microcolonies eliminates the requirement to have skilled analysts and limits the results variability. It also greatly reduces the time to results to 48 h instead of 7-10 days with ISO 11731:2017 while providing statistically equivalent results.",

keywords = "Humans, Legionella, Legionella pneumophila, Legionnaires' Disease/prevention & control, Water, Water Microbiology",

author = "Fanny Passot and Sabine Peslier and Benzinger, {M Joseph} and Jonathan Blackburn and Wesley Thompson and Benjamin Bastin and Audrey Dumont and Sam Dukan",

note = "{\textcopyright} The Author(s) 2022. Published by Oxford University Press on behalf of AOAC INTERNATIONAL.",

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doi = "10.1093/jaoacint/qsac150",

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T2 - AOAC Performance Tested MethodSM #032201

AU - Passot, Fanny

AU - Peslier, Sabine

AU - Benzinger, M Joseph

AU - Blackburn, Jonathan

AU - Thompson, Wesley

AU - Bastin, Benjamin

AU - Dumont, Audrey

AU - Dukan, Sam

N1 - © The Author(s) 2022. Published by Oxford University Press on behalf of AOAC INTERNATIONAL.

PY - 2023/5/3

Y1 - 2023/5/3

N2 - BACKGROUND: Frequent testing for Legionella concentration in water is required by most health risk monitoring organizations worldwide. Domestic hot water and cooling tower water networks must be regularly controlled to prevent Legionnaires' disease, a potentially deadly lung infection. MICA Legionella is the fastest culture-based detection method for all serogroups of Legionella pneumophila, with automatic enumeration in 48 h and no need for confirmation.OBJECTIVE: This study compares the performance and robustness of MICA Legionella with the reference method ISO 11731:2017 for the enumeration of culturable L. pneumophila.METHODS: MICA Legionella and ISO 11731:2017 results were compared for domestic hot water and cooling tower water. Inclusivity and exclusivity were tested on reference and environmental strains. Ruggedness, lot-to-lot consistency, and stability of the reagents kit were also studied.RESULTS: Enumeration of L. pneumophila by MICA Legionella was statistically equivalent to ISO 11731:2017 in both matrixes. In cooling tower waters, MICA Legionella showed better sensitivity than ISO 11731:2017. It presented a 94% sensitivity and a 97% specificity.CONCLUSION: MICA Legionella is a highly sensitive and specific method for culturable L. pneumophila enumeration. It presents, in 48 hours, equivalent or better results than ISO 11731:2017. Its protocol is robust to variations. Its reagents kit is stable for up to 18 months.HIGHLIGHTS: MICA Legionella is a robust and reliable method for the enumeration of culturable L. pneumophila in domestic and cooling tower water. It reduces significantly the number of sample pretreatments required in ISO 11731:2017. Automatic identification and enumeration of L. pneumophila microcolonies eliminates the requirement to have skilled analysts and limits the results variability. It also greatly reduces the time to results to 48 h instead of 7-10 days with ISO 11731:2017 while providing statistically equivalent results.

AB - BACKGROUND: Frequent testing for Legionella concentration in water is required by most health risk monitoring organizations worldwide. Domestic hot water and cooling tower water networks must be regularly controlled to prevent Legionnaires' disease, a potentially deadly lung infection. MICA Legionella is the fastest culture-based detection method for all serogroups of Legionella pneumophila, with automatic enumeration in 48 h and no need for confirmation.OBJECTIVE: This study compares the performance and robustness of MICA Legionella with the reference method ISO 11731:2017 for the enumeration of culturable L. pneumophila.METHODS: MICA Legionella and ISO 11731:2017 results were compared for domestic hot water and cooling tower water. Inclusivity and exclusivity were tested on reference and environmental strains. Ruggedness, lot-to-lot consistency, and stability of the reagents kit were also studied.RESULTS: Enumeration of L. pneumophila by MICA Legionella was statistically equivalent to ISO 11731:2017 in both matrixes. In cooling tower waters, MICA Legionella showed better sensitivity than ISO 11731:2017. It presented a 94% sensitivity and a 97% specificity.CONCLUSION: MICA Legionella is a highly sensitive and specific method for culturable L. pneumophila enumeration. It presents, in 48 hours, equivalent or better results than ISO 11731:2017. Its protocol is robust to variations. Its reagents kit is stable for up to 18 months.HIGHLIGHTS: MICA Legionella is a robust and reliable method for the enumeration of culturable L. pneumophila in domestic and cooling tower water. It reduces significantly the number of sample pretreatments required in ISO 11731:2017. Automatic identification and enumeration of L. pneumophila microcolonies eliminates the requirement to have skilled analysts and limits the results variability. It also greatly reduces the time to results to 48 h instead of 7-10 days with ISO 11731:2017 while providing statistically equivalent results.

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KW - Legionnaires' Disease/prevention & control

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ER -

Validation of MICA Legionella for Enumeration of Legionella pneumophila in sanitary waters and cooling tower waters: AOAC Performance Tested MethodSM #032201

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