Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Vaccination with outer membrane vesicles and the fimbrial protein FlfA offers improved protection against lesions following challenge with Gallibacterium anatis

Research output: Contribution to journalJournal articleResearchpeer-review

  1. The membrane transporter PotE is required for virulence in avian pathogenic Escherichia coli (APEC)

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The occurrence of biofilm in an equine experimental wound model of healing by secondary intention

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Heat resistance in extended-spectrum beta-lactamase-producing Escherichia coli may favor environmental survival in a hospital setting

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. N-acetylcysteine protects ovarian follicles from ischemia-reperfusion injury in xenotransplanted human ovarian tissue

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Effect of whey protein supplementation on sperm quality and fertility in male mice

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Effect of sphingosine-1-phosphate on activation of dormant follicles in murine and human ovarian tissue

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Gallibacterium anatis is an opportunistic poultry pathogen belonging to the Pasteurellaceae family. It has been shown to cause oophoritis, salpingitis and peritonitis in hens, as well as being associated with reduced semen quality in cockerels. Widespread multidrug resistance and substantial antigenic variation among strains of Gallibacterium anatis is a major constraint to treatment with antimicrobials and prevention of infection by vaccination. Novel vaccine strategies targeting G. anatis are therefore necessary. Outer membrane vesicles (OMVs) are nanosized vesicles formed from the outer membrane of Gram-negative bacteria. These vesicles have shown promising potential as both adjuvants and as vaccine candidates against numerous bacterial species. A high vesiculating mutant of G. anatis (G. anatis ΔtolR) has previously been made, enabling production of OMVs in large scale. In this study, we elucidated the potential of G. anatis ΔtolR OMVs as adjuvant for the conserved antigens GtxA-N (the N-terminal part of the RTX like toxin Gallibacterium toxin A) and FlfA (F17-like fimbria), as well as evaluated if combinations of OMVs together with antigens could facilitate cross-protective immunity against three different strains of G. anatis. We showed that ΔtolR OMVs function as an adjuvant for GtxA-N by inducing antigen specific antibody production. However, OMVs in combination with GtxA-N failed to induce protection against lesions after challenge infection. In contrast, vaccination with OMVs in combination with FlfA protected against lesions, especially in the salpinx, caused by two diverse strains of G. anatis, thereby indicating a cross-protective potential. No protection against the third G. anatis strain 7990 could be obtained in any of the experimental settings. In conclusion, ΔtolR OMVs and FlfA could serve as potential future vaccine components againt G. anatis.

Original languageEnglish
JournalVeterinary Microbiology
Volume217
Pages (from-to)104-111
Number of pages8
ISSN0378-1135
DOIs
Publication statusPublished - Apr 2018

ID: 55118261