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UV gradient combined with principal component analysis: highly sensitive and specific high performance liquid chromatography analysis of cosmetic creams

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HPLC has been employed to develop a method for the analysis of cosmetic creams, in particular the compounds hydroquinone, phenol and six preservatives have been studied. UV tuning was optimized as a gradient to achieve lower limits of detection compared to those of a previously validated method. In addition the chromatograms were then exported, aligned and visualized in a principal component analysis (PCA) model. The results were the highly efficient separation of the eight studied compounds. All the compounds showed good linear correlation coefficients (≥ 0.9997), the detection limit was found to be in the range of 15-200 ng/mL, a 10-fold improvement for the preservatives on previous methodology and the average recovery was within limits between 83% and 117% with a relative standard deviation (RSD) less than 3.6% (n=6). The PCA plot was constructed from the UV optimized cosmetic samples chromatograms from real samples, real samples that were spiked and quality controls. Quality controls contained the eight compounds and showed complete clustering in the PCA and three spiked samples containing six to seven toxic components clustered in the same quadrant. The method is highly sensitive and its potential use as a method that could be employed in the control of cosmetics, particularly those containing banned or suspected toxic additives, has been demonstrated.

Original languageEnglish
JournalJournal of Chromatography
Volume1228
Pages (from-to)324-8
Number of pages5
ISSN0021-9673
DOIs
Publication statusPublished - 9 Mar 2012
Externally publishedYes

    Research areas

  • Chromatography, High Pressure Liquid/methods, Cluster Analysis, Cosmetics/chemistry, Hydroquinones/analysis, Parabens/analysis, Phenol/analysis, Principal Component Analysis/methods, Reproducibility of Results, Sensitivity and Specificity, Ultraviolet Rays

ID: 54961345