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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Urokinase receptor mRNA level and gene transcription are strongly and rapidly increased by phorbol myristate acetate in human monocyte-like U937 cells

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  1. TAFI deficiency causes maladaptive vascular remodeling after hemophilic joint bleeding

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  2. CCL2/MCP-1 signaling drives extracellular matrix turnover by diverse macrophage subsets

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  3. Immune regulation by fibroblasts in tissue injury depends on uPARAP-mediated uptake of collectins

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  4. uPARAP/Endo180 receptor is a gatekeeper of VEGFR-2/VEGFR-3 heterodimerisation during pathological lymphangiogenesis

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  5. Defective TAFI activation in hemophilia A mice is a major contributor to joint bleeding

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We have studied the effect of the tumor promotor phorbol myristate acetate (PMA) on the level of mRNA for the receptor for urokinase-type plasminogen activator (u-PAR) in the human monocyte-like cell line U937. PMA causes an early increase in the u-PAR mRNA level which reaches a maximal 50-fold enhancement after 24 h of treatment. Half-maximal stimulation occurs at approximately 5 nM PMA. The effect is observed only with phorbol esters that also act as tumor promotors. The protein synthesis inhibitor cycloheximide (10 micrograms/ml) also increases the level of u-PAR mRNA. Nuclear run-on experiments show a time-dependent increase in the u-PAR gene transcription rate after exposure of the cells to PMA. The PMA-induced increase in u-PAR mRNA is paralleled by a time-dependent increase in u-PAR protein as detected by cross-linking studies with radiolabeled ligand. We conclude that PMA stimulates transcription of the u-PAR gene in U937 cells, and this is responsible at least in part for the accumulation of the u-PAR mRNA and for the subsequent increase in urokinase-binding capacity.

Original languageEnglish
JournalThe journal of biological chemistry
Volume266
Issue number8
Pages (from-to)5177-81
Number of pages5
ISSN0021-9258
Publication statusPublished - 15 Mar 1991

    Research areas

  • Blotting, Northern, Cell Line, Cycloheximide, DNA, Humans, Monocytes, RNA, Messenger, Receptors, Cell Surface, Receptors, Urokinase Plasminogen Activator, Tetradecanoylphorbol Acetate, Transcription, Genetic, Urokinase-Type Plasminogen Activator

ID: 46434653