Up-regulation of alpha1A-adrenoceptors in rat mesenteric artery involves intracellular signal pathways

Yong-Xiao Cao; Cang-Bao Xu; Guo-Gang Luo; Lars Edvinsson

doi:10.1111/j.1742-7843.2006.pto_240.x

Up-regulation of alpha1A-adrenoceptors in rat mesenteric artery involves intracellular signal pathways

Yong-Xiao Cao, Cang-Bao Xu, Guo-Gang Luo, Lars Edvinsson

13 Citations (Scopus)

Abstract

The aim of the present study was to investigate if there is an altered expression of alpha-adrenoceptors during organ culture of rat mesenteric artery segments by using a sensitive pharmacological method and molecular biological techniques. Noradrenalin (NA) induced contraction via alpha1-adrenoceptors. The contraction and alpha1A-adrenoceptor mRNA levels were elevated during organ culture. Transcriptional inhibitor actinomycin D, translational inhibitor cycloheximide, protein kinase C inhibitors (staurosporine and RO31-8220) and mitogen-activated protein kinase (MAPK) pathway inhibitors (SB386023, U0126 and SB239063) prevented the increase in NA-induced contractions. The amount of alpha1A-adrenoceptor mRNA was significantly lower in the artery segments cultured for 1 day in the presence of specific MAPK extracellular signal-regulated protein kinase1/2 pathway inhibitor SB386023 than that of the cultured controls. SB386023 did not affect alpha2-adrenoceptor mRNA level. Our results suggest that the up-regulation of alpha1A-adrenoceptors involves transcription and intracellular signal transduction via the protein kinase C and the ERK 1/2 pathways.

Original language	English
Journal	Basic & clinical pharmacology & toxicology
Volume	98
Issue number	1
Pages (from-to)	61-7
Number of pages	7
ISSN	1742-7835
DOIs	https://doi.org/10.1111/j.1742-7843.2006.pto_240.x
Publication status	Published - Jan 2006
Externally published	Yes

Keywords

Adrenergic alpha-Agonists
Animals
Enzyme Inhibitors
Imidazoles
Mesenteric Artery, Superior
Mitogen-Activated Protein Kinases
Norepinephrine
Organ Culture Techniques
Phosphorylation
Protein Kinase C
Pyrimidines
RNA, Messenger
Rats
Rats, Sprague-Dawley
Receptors, Adrenergic, alpha-1
Signal Transduction
Staurosporine
Up-Regulation
Vasoconstriction

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10.1111/j.1742-7843.2006.pto_240.x

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title = "Up-regulation of alpha1A-adrenoceptors in rat mesenteric artery involves intracellular signal pathways",

abstract = "The aim of the present study was to investigate if there is an altered expression of alpha-adrenoceptors during organ culture of rat mesenteric artery segments by using a sensitive pharmacological method and molecular biological techniques. Noradrenalin (NA) induced contraction via alpha1-adrenoceptors. The contraction and alpha1A-adrenoceptor mRNA levels were elevated during organ culture. Transcriptional inhibitor actinomycin D, translational inhibitor cycloheximide, protein kinase C inhibitors (staurosporine and RO31-8220) and mitogen-activated protein kinase (MAPK) pathway inhibitors (SB386023, U0126 and SB239063) prevented the increase in NA-induced contractions. The amount of alpha1A-adrenoceptor mRNA was significantly lower in the artery segments cultured for 1 day in the presence of specific MAPK extracellular signal-regulated protein kinase1/2 pathway inhibitor SB386023 than that of the cultured controls. SB386023 did not affect alpha2-adrenoceptor mRNA level. Our results suggest that the up-regulation of alpha1A-adrenoceptors involves transcription and intracellular signal transduction via the protein kinase C and the ERK 1/2 pathways.",

keywords = "Adrenergic alpha-Agonists, Animals, Enzyme Inhibitors, Imidazoles, Mesenteric Artery, Superior, Mitogen-Activated Protein Kinases, Norepinephrine, Organ Culture Techniques, Phosphorylation, Protein Kinase C, Pyrimidines, RNA, Messenger, Rats, Rats, Sprague-Dawley, Receptors, Adrenergic, alpha-1, Signal Transduction, Staurosporine, Up-Regulation, Vasoconstriction",

author = "Yong-Xiao Cao and Cang-Bao Xu and Guo-Gang Luo and Lars Edvinsson",

year = "2006",

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language = "English",

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T1 - Up-regulation of alpha1A-adrenoceptors in rat mesenteric artery involves intracellular signal pathways

AU - Cao, Yong-Xiao

AU - Xu, Cang-Bao

AU - Luo, Guo-Gang

AU - Edvinsson, Lars

PY - 2006/1

Y1 - 2006/1

N2 - The aim of the present study was to investigate if there is an altered expression of alpha-adrenoceptors during organ culture of rat mesenteric artery segments by using a sensitive pharmacological method and molecular biological techniques. Noradrenalin (NA) induced contraction via alpha1-adrenoceptors. The contraction and alpha1A-adrenoceptor mRNA levels were elevated during organ culture. Transcriptional inhibitor actinomycin D, translational inhibitor cycloheximide, protein kinase C inhibitors (staurosporine and RO31-8220) and mitogen-activated protein kinase (MAPK) pathway inhibitors (SB386023, U0126 and SB239063) prevented the increase in NA-induced contractions. The amount of alpha1A-adrenoceptor mRNA was significantly lower in the artery segments cultured for 1 day in the presence of specific MAPK extracellular signal-regulated protein kinase1/2 pathway inhibitor SB386023 than that of the cultured controls. SB386023 did not affect alpha2-adrenoceptor mRNA level. Our results suggest that the up-regulation of alpha1A-adrenoceptors involves transcription and intracellular signal transduction via the protein kinase C and the ERK 1/2 pathways.

AB - The aim of the present study was to investigate if there is an altered expression of alpha-adrenoceptors during organ culture of rat mesenteric artery segments by using a sensitive pharmacological method and molecular biological techniques. Noradrenalin (NA) induced contraction via alpha1-adrenoceptors. The contraction and alpha1A-adrenoceptor mRNA levels were elevated during organ culture. Transcriptional inhibitor actinomycin D, translational inhibitor cycloheximide, protein kinase C inhibitors (staurosporine and RO31-8220) and mitogen-activated protein kinase (MAPK) pathway inhibitors (SB386023, U0126 and SB239063) prevented the increase in NA-induced contractions. The amount of alpha1A-adrenoceptor mRNA was significantly lower in the artery segments cultured for 1 day in the presence of specific MAPK extracellular signal-regulated protein kinase1/2 pathway inhibitor SB386023 than that of the cultured controls. SB386023 did not affect alpha2-adrenoceptor mRNA level. Our results suggest that the up-regulation of alpha1A-adrenoceptors involves transcription and intracellular signal transduction via the protein kinase C and the ERK 1/2 pathways.

KW - Adrenergic alpha-Agonists

KW - Animals

KW - Enzyme Inhibitors

KW - Imidazoles

KW - Mesenteric Artery, Superior

KW - Mitogen-Activated Protein Kinases

KW - Norepinephrine

KW - Organ Culture Techniques

KW - Phosphorylation

KW - Protein Kinase C

KW - Pyrimidines

KW - RNA, Messenger

KW - Rats

KW - Rats, Sprague-Dawley

KW - Receptors, Adrenergic, alpha-1

KW - Signal Transduction

KW - Staurosporine

KW - Up-Regulation

KW - Vasoconstriction

UR - https://www.scopus.com/pages/publications/33645097429

U2 - 10.1111/j.1742-7843.2006.pto_240.x

DO - 10.1111/j.1742-7843.2006.pto_240.x

M3 - Journal article

C2 - 16433893

SN - 1742-7835

VL - 98

SP - 61

EP - 67

JO - Basic & clinical pharmacology & toxicology

JF - Basic & clinical pharmacology & toxicology

IS - 1

ER -

Up-regulation of alpha1A-adrenoceptors in rat mesenteric artery involves intracellular signal pathways

Abstract

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