Ultra-High Temperature Treatment and Storage of Infant Formula Induces Dietary Protein Modifications, Gut Dysfunction, and Inflammation in Preterm Pigs

Jing Sun, Halise Gül Akıllıoğlu, Karoline Aasmul-Olsen, Yuhui Ye, Pernille Lund, Xiao Zhao, Anders Brunse, Christian Fiil Nielsen, Dereck E W Chatterton, Per Torp Sangild, Marianne N Lund, Stine Brandt Bering*

*Corresponding author for this work

Abstract

SCOPE: Ready-to-feed liquid infant formula is increasingly used for preterm infants when human milk is unavailable. These formulas are sterilized by ultra-high temperature treatment, but heating and storage may reduce bioactivity and increase formation of Maillard reaction products with potential negative consequences for immature newborns.

METHODS AND RESULTS: Using preterm pigs as a model for sensitive newborn infants, the study tests the intestinal responses of feeding experimental liquid formula within 5 days. A pasteurized formula (PAST) with the same nutrient composition but less protein modifications serves as control to ultra-high temperature-treated formula without (UHT) and with prolonged storage (SUHT). Relative to PAST, UHT contains lower levels of lactoferrin and IgG. Additional storage (40 °C, 60 days, SUHT) reduces antimicrobial capacity and increases non-reducible protein aggregates and Maillard reaction products (up to 13-fold). Pigs fed SUHT have more diarrhea and show signs of intestinal inflammation (necrotizing enterocolitis) compared with pigs fed PAST and UHT. These clinical effects are accompanied by accumulation of Maillard reaction products, protein cross-links, and inflammatory responses in the gut.

CONCLUSION: The results demonstrate that feeding UHT infant formulas, particularly after prolonged storage, adversely affects gut maturation and function in preterm pigs used as a model of preterm infants.

Original languageEnglish
Article number2200132
JournalMolecular Nutrition & Food Research
Volume66
Issue number20
Pages (from-to)e2200132
ISSN1613-4125
DOIs
Publication statusPublished - Oct 2022

Keywords

  • Animals
  • Animals, Newborn
  • Glycation End Products, Advanced
  • Humans
  • Immunoglobulin G
  • Infant
  • Infant Formula
  • Infant, Newborn
  • Infant, Premature
  • Inflammation
  • Intestines/physiology
  • Lactoferrin
  • Protein Aggregates
  • Swine
  • Temperature

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