Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

True Chromogranin A concentrations in plasma from patients with small intestinal neuroendocrine tumours

Research output: Contribution to journalJournal articleResearchpeer-review

  1. 3D analysis of the myenteric plexus of the human bowel by X-ray phase-contrast tomography - a future method?

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Colonoscopy adverse events: are we getting the full picture?

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Pathophysiological-based treatments of complications of cirrhosis

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Oral D/L-3-Hydroxybutyrate stimulates cholecystokinin and insulin secretion and slows gastric emptying in healthy males

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Bilio-enteric flow and plasma concentrations of bile acids after gastric bypass and sleeve gastrectomy

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Effect of insulin on natriuretic peptide gene expression in porcine heart

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Objective: The incidence of enteropancreatic neuroendocrine tumours (NET) is increasing. Chromogranin A (CgA) in plasma is a marker in patients suspected of NET tumours. CgA, however, is a precursor protein subjected to cellular processing that challenges quantitation and hence the use of CgA in diagnostics.Materials and methods: CgA concentrations in plasma sampled from 130 well-characterized patients with small intestinal NETs and from 30 healthy subjects were measured with eight commercial CgA kits, an in-house radioimmunoassay (RIA) and a processing-independent assay (PIA). For the evaluation of diagnostic accuracy, we performed regression analyses and plotted receiver-operating characteristic curves (ROC). The specificity was further assessed by size chromatography.Results: Five commercial assays (Thermo-Fisher, DRG Diagnostics, Eurodiagnostica (RIA and ELISA), and Phoenix), displayed a diagnostic accuracy with area under the curve (AUC) values >0.90, whereas three immunoassays (Yanaihara, CisBio RIA, and CisBio ELISA) discriminated poorly between disease stages (AUC: 0.60-0.78). Compared with the in-house assays, however, even the most accurate commercial immunoassay still missed patients with metastatic disease. Chromatography showed non-uniform patterns of large and small CgA fragments in plasma.Conclusion: Available commercial immunoassays measure CgA in plasma with gross variability. Three commercial CgA immunoassays discriminate so poorly between health and disease that they should not be used. The highest diagnostic accuracy was obtained with processing-independent measurement of total CgA concentrations in plasma.

Original languageEnglish
JournalScandinavian Journal of Gastroenterology
Volume55
Issue number5
Pages (from-to)565-573
Number of pages9
ISSN0036-5521
DOIs
Publication statusPublished - 2020

ID: 60068656