Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Transfection of Tumor-Infiltrating T Cells with mRNA Encoding CXCR2

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Determination of Binding Kinetics of Intrinsically Disordered Proteins by Surface Plasmon Resonance

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Analysis of Mass Cytometry Data

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Assessment of Peptidylarginine Deiminase Activity by ELISA Using Human Fibrinogen as Substrate

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Full-Length Open Reading Frame Amplification of Hepatitis C Virus

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Capsid-like particles decorated with the SARS-CoV-2 receptor-binding domain elicit strong virus neutralization activity

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Lynch syndrome-associated epithelial ovarian cancer and its immunological profile

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Adoptive T-cell therapy based on the infusion of patient's own immune cells after ex vivo culturing is among the most potent forms of personalized treatment among recent clinical developments for the treatment of cancer. However, despite high rates of successful initial clinical responses, only about 20 % of patients with metastatic melanoma treated with tumor-infiltrating lymphocytes (TILs) enter complete and long-term regression, with the majority either relapsing after initial partial regression or not benefiting at all. Previous studies have shown a positive correlation between the number infused T cells migrating to the tumor and the clinical response, but also that only a small fraction of adoptively transferred T cells reach the tumor site. In this chapter, we describe a protocol for transfection of TILs with mRNA encoding the chemokine receptor CXCR2 transiently redirecting and improving TILs migration toward tumor-secreted chemokines in vitro.

Original languageEnglish
JournalMethods in molecular biology
Volume1428
Pages (from-to)261-76
Number of pages16
ISSN1064-3745
DOIs
Publication statusPublished - 2016

    Research areas

  • Journal Article

ID: 49639943