Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Trans-ethnic gut microbiota signatures of type 2 diabetes in Denmark and India

Research output: Contribution to journalJournal articleResearchpeer-review

  1. The global dissemination of hospital clones of Enterococcus faecium

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Whole blood co-expression modules associate with metabolic traits and type 2 diabetes: an IMI-DIRECT study

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. High frequency of pathogenic germline variants within homologous recombination repair in patients with advanced cancer

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Elevated polygenic burden for autism is associated with differential DNA methylation at birth

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Once-weekly subcutaneous semaglutide treatment for persons with type 2 diabetes: Real-world data from a diabetes out-patient clinic

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. 2021 ESC Guidelines on cardiovascular disease prevention in clinical practice

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Influence of educational level on test and treatment for incident hypothyroidism

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Camila Alvarez-Silva
  • Alireza Kashani
  • Tue Haldor Hansen
  • Nishal Kumar Pinna
  • Ranjit Mohan Anjana
  • Anirban Dutta
  • Shruti Saxena
  • Julie Støy
  • Ulla Kampmann
  • Trine Nielsen
  • Torben Jørgensen
  • Visvanathan Gnanaprakash
  • Rameshkumar Gnanavadivel
  • Aswath Sukumaran
  • Coimbatore Subramanian Shanthi Rani
  • Kristine Færch
  • Venkatesan Radha
  • Muthuswamy Balasubramanyam
  • Gopinath Balakrish Nair
  • Bhabatosh Das
  • Henrik Vestergaard
  • Torben Hansen
  • Sharmila Shekhar Mande
  • Viswanathan Mohan
  • Manimozhiyan Arumugam
  • Oluf Pedersen
View graph of relations

BACKGROUND: Type 2 diabetes (T2D), a multifactorial disease influenced by host genetics and environmental factors, is the most common endocrine disease. Several studies have shown that the gut microbiota as a close-up environmental mediator influences host physiology including metabolism. The aim of the present study is to examine the compositional and functional potential of the gut microbiota across individuals from Denmark and South India with a focus on T2D. Many earlier studies have investigated the microbiome aspects of T2D, and it has also been anticipated that such microbial associations would be dependent on diet and ethnic origin. However, there has been no large scale trans-ethnic microbiome study earlier in this direction aimed at evaluating any "universal" microbiome signature of T2D.

METHODS: 16S ribosomal RNA gene amplicon sequencing was performed on stool samples from 279 Danish and 294 Indian study participants. Any differences between the gut microbiota of both populations were explored using diversity measures and negative binomial Wald tests. Study samples were stratified to discover global and country-specific microbial signatures for T2D and treatment with the anti-hyperglycemic drug, metformin. To identify taxonomical and functional signatures of the gut microbiota for T2D and metformin treatment, we used alpha and beta diversity measures and differential abundances analysis, comparing metformin-naive T2D patients, metformin-treated T2D patients, and normoglycemic individuals.

RESULTS: Overall, the gut microbial communities of Danes and Indians are compositionally very different. By analyzing the combined study materials, we identify microbial taxonomic and functional signatures for T2D and metformin treatment. T2D patients have an increased relative abundance of two operational taxonomic units (OTUs) from the Lachnospiraceae family, and a decreased abundance of Subdoligranulum and Butyricicoccus. Studying each population per se, we identified T2D-related microbial changes at the taxonomic level within the Danish population only. Alpha diversity indices show that there is no significant difference between normoglycemic individuals and metformin-naive T2D patients, whereas microbial richness is significantly decreased in metformin-treated T2D patients compared to metformin-naive T2D patients and normoglycemic individuals. Enrichment of two OTUs from Bacteroides and depletion of Faecalibacterium constitute a trans-ethnic signature of metformin treatment.

CONCLUSIONS: We demonstrate major compositional differences of the gut microbiota between Danish and South Indian individuals, some of which may relate to differences in ethnicity, lifestyle, and demography. By comparing metformin-naive T2D patients and normoglycemic individuals, we identify T2D-related microbiota changes in the Danish and Indian study samples. In the present trans-ethnic study, we confirm that metformin changes the taxonomic profile and functional potential of the gut microbiota.

Original languageEnglish
Article number37
JournalGenome Medicine
Volume13
Issue number1
Pages (from-to)37
ISSN1756-994X
DOIs
Publication statusPublished - 3 Mar 2021

    Research areas

  • Danes, Gut microbiota, Indians, Metformin, Populations, Trans-ethnic, Type 2 diabetes

ID: 63845325