Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Trans-ethnic gut microbial signatures of prediabetic subjects from India and Denmark

Research output: Contribution to journalJournal articleResearchpeer-review

  1. Trans-ethnic gut microbiota signatures of type 2 diabetes in Denmark and India

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Whole blood co-expression modules associate with metabolic traits and type 2 diabetes: an IMI-DIRECT study

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. High frequency of pathogenic germline variants within homologous recombination repair in patients with advanced cancer

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Elevated polygenic burden for autism is associated with differential DNA methylation at birth

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. The day-night pattern of colonic contractility is not impaired in type 1 diabetes and distal symmetric polyneuropathy

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Watching, keeping and squeezing time to lose weight: implications of time-restricted eating in daily life

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Influence of educational level on test and treatment for incident hypothyroidism

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Three different approaches to delimitation of functional somatic disorders: DanFunD

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Taking no for an answer. Nurses' consultations with people with cardiac disease about rehabilitation: A qualitative study

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Nishal Kumar Pinna
  • Ranjit Mohan Anjana
  • Shruti Saxena
  • Anirban Dutta
  • Visvanathan Gnanaprakash
  • Gnanavadivel Rameshkumar
  • Sukumaran Aswath
  • Srividhya Raghavan
  • Coimbatore Subramanian Shanthi Rani
  • Venkatesan Radha
  • Muthuswamy Balasubramanyam
  • Archana Pant
  • Trine Nielsen
  • Torben Jørgensen
  • Kristine Færch
  • Alireza Kashani
  • Maria Camila Alvarez Silva
  • Henrik Vestergaard
  • Tue Haldor Hansen
  • Torben Hansen
  • Manimozhiyan Arumugam
  • Gopinath Balakrish Nair
  • Bhabatosh Das
  • Oluf Pedersen
  • Viswanathan Mohan
  • Sharmila Shekhar Mande
View graph of relations

BACKGROUND: Recent studies have indicated an association of gut microbiota and microbial metabolites with type 2 diabetes mellitus (T2D). However, large-scale investigation of the gut microbiota of "prediabetic" (PD) subjects has not been reported. Identifying robust gut microbiome signatures of prediabetes and characterizing early prediabetic stages is important for the understanding of disease development and could be crucial in early diagnosis and prevention.

METHODS: The current study performed amplification and sequencing on the variable regions (V1-V5) of the 16S rRNA genes to profile and compare gut microbiota of prediabetic individuals (N = 262) with normoglycemic individuals (N = 275) from two cohorts in India and Denmark. Similarly, fasting serum inflammatory biomarkers were profiled from the study participants.

RESULTS: After correcting for strong country-specific cohort effect, 16 operational taxonomic units (OTUs) including members from the genera Prevotella9, Phascolarctobacterium, Barnesiella, Flavonifractor, Tyzzerella_4, Bacteroides, Faecalibacterium, and Agathobacter were identified as enriched in normoglycaemic subjects with respect to the subjects with prediabetes using a negative binomial Wald test. We also identified 144 OTUs enriched in the prediabetic subjects, which included members from the genera Megasphaera, Streptococcus, Prevotella9, Alistipes, Mitsuokella, Escherichia/Shigella, Prevotella2, Vibrio, Lactobacillus, Alloprevotella, Rhodococcus, and Klebsiella. Comparative analyses of relative abundance of bacterial taxa revealed that the Streptococcus, Escherichia/Shigella, Prevotella2, Vibrio, and Alloprevotella OTUs exhibited more than fourfold enrichment in the gut microbiota of prediabetic subjects. When considering subjects from the two geographies separately, we were able to identify additional gut microbiome signatures of prediabetes. The study reports a probable association of Megasphaera OTU(s) with impaired glucose tolerance, which is significantly pronounced in Indian subjects. While the overall results confirm a state of proinflammation as early as in prediabetes, the Indian cohort exhibited a characteristic pattern of abundance of inflammatory markers indicating low-grade intestinal inflammation at an overall population level, irrespective of glycemic status.

CONCLUSIONS: The results present trans-ethnic gut microbiome and inflammation signatures associated with prediabetes, in Indian and Danish populations. The identified associations may be explored further as potential early indicators for individuals at risk of dysglycemia.

Original languageEnglish
Article number36
JournalGenome Medicine
Volume13
Issue number1
Pages (from-to)36
ISSN1756-994X
DOIs
Publication statusPublished - 3 Mar 2021

ID: 63845173