TPO-immunfarvning af den solitaere, kolde thyreoideaknude

M Lange; M Blichert-Toft; L H Christensen; M Brandt; S B Sneppen; J Ravnsbaek; C L Mollerup; L Strange; F Jensen; J Kirkegaard; H S Hansen; S S Sørensen; U Feldt-Rasmussen

TPO-immunfarvning af den solitaere, kolde thyreoideaknude

Translated title of the contribution: TPO immunostaining of the solitary, cold thyroid nodules

M Lange, M Blichert-Toft, L H Christensen, M Brandt, S B Sneppen, J Ravnsbaek, C L Mollerup, L Strange, F Jensen, J Kirkegaard, H S Hansen, S S Sørensen, U Feldt-Rasmussen

1 Citation (Scopus)

Abstract

INTRODUCTION: The chance of malignancy in scintigraphically cold thyroid nodules is 2-24%. Differentiation between malignant and benign cytology is difficult. The aim of this study was to evaluate the ability of immunostaining (MoAB47--raised against thyroid peroxidase (TPO)) to differentiate between malignant and benign cells taken from cold thyroid nodules by fine needle aspiration biopsy (FNAB) in order to reduce the number of unnecessary thyroid operations.

MATERIALS AND METHODS: One hundred and eighty-one patients (150 female) with a scintigraphically cold, solitary thyroid nodule were entered between 1993 and 1996. Fifty-seven were excluded for various reasons. Material removed by FNAB was stained with MoAB47 and routine staining. Staining of 80% or more of the cells was considered benign, less than 80% was considered malignant. Routine staining of operatively removed material was used as the final diagnosis.

RESULTS: A pattern with negative TPO staining was found in all lesions that were subsequently proved to be malignant. In all but one, the lesions subsequently diagnosed as being benign stained positive for TPO. The sensitivity and specificity were respectively 1.0 and 0.99.

CONCLUSION: TPO immunostaining of material removed by FNAB is a powerful tool in the differentiation between benign and malignant tumours.

Translated title of the contribution	TPO immunostaining of the solitary, cold thyroid nodules
Original language	Danish
Journal	Ugeskrift for Laeger
Volume	163
Issue number	32
Pages (from-to)	4198-201
Number of pages	4
ISSN	0041-5782
Publication status	Published - 6 Aug 2001
Externally published	Yes

Cite this

@article{c15003fd2e504612894e5e7797e513bb,

title = "TPO-immunfarvning af den solitaere, kolde thyreoideaknude",

abstract = "INTRODUCTION: The chance of malignancy in scintigraphically cold thyroid nodules is 2-24\%. Differentiation between malignant and benign cytology is difficult. The aim of this study was to evaluate the ability of immunostaining (MoAB47--raised against thyroid peroxidase (TPO)) to differentiate between malignant and benign cells taken from cold thyroid nodules by fine needle aspiration biopsy (FNAB) in order to reduce the number of unnecessary thyroid operations.MATERIALS AND METHODS: One hundred and eighty-one patients (150 female) with a scintigraphically cold, solitary thyroid nodule were entered between 1993 and 1996. Fifty-seven were excluded for various reasons. Material removed by FNAB was stained with MoAB47 and routine staining. Staining of 80\% or more of the cells was considered benign, less than 80\% was considered malignant. Routine staining of operatively removed material was used as the final diagnosis.RESULTS: A pattern with negative TPO staining was found in all lesions that were subsequently proved to be malignant. In all but one, the lesions subsequently diagnosed as being benign stained positive for TPO. The sensitivity and specificity were respectively 1.0 and 0.99.CONCLUSION: TPO immunostaining of material removed by FNAB is a powerful tool in the differentiation between benign and malignant tumours.",

keywords = "Adolescent, Adult, Aged, Biopsy, Needle, Female, Humans, Immunohistochemistry/methods, Iodide Peroxidase/immunology, Male, Middle Aged, Radionuclide Imaging, Sensitivity and Specificity, Staining and Labeling/methods, Thyroid Nodule/diagnostic imaging",

author = "M Lange and M Blichert-Toft and Christensen, \{L H\} and M Brandt and Sneppen, \{S B\} and J Ravnsbaek and Mollerup, \{C L\} and L Strange and F Jensen and J Kirkegaard and Hansen, \{H S\} and S{\o}rensen, \{S S\} and U Feldt-Rasmussen",

year = "2001",

month = aug,

day = "6",

language = "Dansk",

volume = "163",

pages = "4198--201",

journal = "Ugeskrift for Laeger",

issn = "0041-5782",

publisher = "Almindelige Danske Laegeforening",

number = "32",

}

TY - JOUR

T1 - TPO-immunfarvning af den solitaere, kolde thyreoideaknude

AU - Lange, M

AU - Blichert-Toft, M

AU - Christensen, L H

AU - Brandt, M

AU - Sneppen, S B

AU - Ravnsbaek, J

AU - Mollerup, C L

AU - Strange, L

AU - Jensen, F

AU - Kirkegaard, J

AU - Hansen, H S

AU - Sørensen, S S

AU - Feldt-Rasmussen, U

PY - 2001/8/6

Y1 - 2001/8/6

N2 - INTRODUCTION: The chance of malignancy in scintigraphically cold thyroid nodules is 2-24%. Differentiation between malignant and benign cytology is difficult. The aim of this study was to evaluate the ability of immunostaining (MoAB47--raised against thyroid peroxidase (TPO)) to differentiate between malignant and benign cells taken from cold thyroid nodules by fine needle aspiration biopsy (FNAB) in order to reduce the number of unnecessary thyroid operations.MATERIALS AND METHODS: One hundred and eighty-one patients (150 female) with a scintigraphically cold, solitary thyroid nodule were entered between 1993 and 1996. Fifty-seven were excluded for various reasons. Material removed by FNAB was stained with MoAB47 and routine staining. Staining of 80% or more of the cells was considered benign, less than 80% was considered malignant. Routine staining of operatively removed material was used as the final diagnosis.RESULTS: A pattern with negative TPO staining was found in all lesions that were subsequently proved to be malignant. In all but one, the lesions subsequently diagnosed as being benign stained positive for TPO. The sensitivity and specificity were respectively 1.0 and 0.99.CONCLUSION: TPO immunostaining of material removed by FNAB is a powerful tool in the differentiation between benign and malignant tumours.

AB - INTRODUCTION: The chance of malignancy in scintigraphically cold thyroid nodules is 2-24%. Differentiation between malignant and benign cytology is difficult. The aim of this study was to evaluate the ability of immunostaining (MoAB47--raised against thyroid peroxidase (TPO)) to differentiate between malignant and benign cells taken from cold thyroid nodules by fine needle aspiration biopsy (FNAB) in order to reduce the number of unnecessary thyroid operations.MATERIALS AND METHODS: One hundred and eighty-one patients (150 female) with a scintigraphically cold, solitary thyroid nodule were entered between 1993 and 1996. Fifty-seven were excluded for various reasons. Material removed by FNAB was stained with MoAB47 and routine staining. Staining of 80% or more of the cells was considered benign, less than 80% was considered malignant. Routine staining of operatively removed material was used as the final diagnosis.RESULTS: A pattern with negative TPO staining was found in all lesions that were subsequently proved to be malignant. In all but one, the lesions subsequently diagnosed as being benign stained positive for TPO. The sensitivity and specificity were respectively 1.0 and 0.99.CONCLUSION: TPO immunostaining of material removed by FNAB is a powerful tool in the differentiation between benign and malignant tumours.

KW - Adolescent

KW - Adult

KW - Aged

KW - Biopsy, Needle

KW - Female

KW - Humans

KW - Immunohistochemistry/methods

KW - Iodide Peroxidase/immunology

KW - Male

KW - Middle Aged

KW - Radionuclide Imaging

KW - Sensitivity and Specificity

KW - Staining and Labeling/methods

KW - Thyroid Nodule/diagnostic imaging

UR - https://www.scopus.com/pages/publications/0035817567

M3 - Tidsskriftartikel

C2 - 11510238

SN - 0041-5782

VL - 163

SP - 4198

EP - 4201

JO - Ugeskrift for Laeger

JF - Ugeskrift for Laeger

IS - 32

ER -

TPO-immunfarvning af den solitaere, kolde thyreoideaknude

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