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Tissue to plasma capillary permeability of 131I-albumin in the perfused rabbit ear

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@article{117ec64c3b9f42a78b1aa32c8b2276b2,
title = "Tissue to plasma capillary permeability of 131I-albumin in the perfused rabbit ear",
abstract = "The tissue to plasma transfer of 131I-albumin was recorded in perfused rabbit ears (n = 6) following equilibration for 24 hr. 125I-fibrinogen served as the plasma marker, and was introduced intravenously 15 min before clamping. The ears were rollerpump perfused with isotonic diluted plasma at a constant rate of (mean +/- SD) 5.1 +/- 1.5 ml (min.100 g)-1. The mean extravascular albumin distribution volume was 12.4 +/- 1.1 ml.100 g-1, and the fibrinogen volume (plasma volume in tissue) was 3.1 +/- 0.4 ml.100 g-1 as determined from biopsies of the contralateral ear. The initial transfer of albumin was marked, and occurred at rates corresponding to a unidirectional clearance (Cl(0)) of 0.068 +/- 0.012 ml (min.100 g)-1. However, with a reduction of mean interstitial albumin tracer content of no more than 4%, net transport decreased to reach slowly declining levels 5 to 10 times lower within 10 min of continued perfusion. The decrease was considered due to rapid exhaustion of a small interstitial pool of tracer immediately adjacent to the exchange vessel membrane, followed by an increasingly retarded outwash from more distant areas. The results suggest a bimodal structural resistance to albumin movement: a relatively low resistance in the capillary membrane, and a considerable restriction to albumin transport located within the interstitial space.",
keywords = "Animals, Biomarkers, Biopsy, Capillary Permeability, Capillary Resistance, Ear, Fibrinogen, Iodine Radioisotopes, Rabbits, Serum Albumin, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't",
author = "Lasse Bent-Hansen and Svendsen, {J H}",
year = "1991",
month = mar,
language = "English",
volume = "41",
pages = "141--8",
journal = "Microvascular Research",
issn = "0026-2862",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Tissue to plasma capillary permeability of 131I-albumin in the perfused rabbit ear

AU - Bent-Hansen, Lasse

AU - Svendsen, J H

PY - 1991/3

Y1 - 1991/3

N2 - The tissue to plasma transfer of 131I-albumin was recorded in perfused rabbit ears (n = 6) following equilibration for 24 hr. 125I-fibrinogen served as the plasma marker, and was introduced intravenously 15 min before clamping. The ears were rollerpump perfused with isotonic diluted plasma at a constant rate of (mean +/- SD) 5.1 +/- 1.5 ml (min.100 g)-1. The mean extravascular albumin distribution volume was 12.4 +/- 1.1 ml.100 g-1, and the fibrinogen volume (plasma volume in tissue) was 3.1 +/- 0.4 ml.100 g-1 as determined from biopsies of the contralateral ear. The initial transfer of albumin was marked, and occurred at rates corresponding to a unidirectional clearance (Cl(0)) of 0.068 +/- 0.012 ml (min.100 g)-1. However, with a reduction of mean interstitial albumin tracer content of no more than 4%, net transport decreased to reach slowly declining levels 5 to 10 times lower within 10 min of continued perfusion. The decrease was considered due to rapid exhaustion of a small interstitial pool of tracer immediately adjacent to the exchange vessel membrane, followed by an increasingly retarded outwash from more distant areas. The results suggest a bimodal structural resistance to albumin movement: a relatively low resistance in the capillary membrane, and a considerable restriction to albumin transport located within the interstitial space.

AB - The tissue to plasma transfer of 131I-albumin was recorded in perfused rabbit ears (n = 6) following equilibration for 24 hr. 125I-fibrinogen served as the plasma marker, and was introduced intravenously 15 min before clamping. The ears were rollerpump perfused with isotonic diluted plasma at a constant rate of (mean +/- SD) 5.1 +/- 1.5 ml (min.100 g)-1. The mean extravascular albumin distribution volume was 12.4 +/- 1.1 ml.100 g-1, and the fibrinogen volume (plasma volume in tissue) was 3.1 +/- 0.4 ml.100 g-1 as determined from biopsies of the contralateral ear. The initial transfer of albumin was marked, and occurred at rates corresponding to a unidirectional clearance (Cl(0)) of 0.068 +/- 0.012 ml (min.100 g)-1. However, with a reduction of mean interstitial albumin tracer content of no more than 4%, net transport decreased to reach slowly declining levels 5 to 10 times lower within 10 min of continued perfusion. The decrease was considered due to rapid exhaustion of a small interstitial pool of tracer immediately adjacent to the exchange vessel membrane, followed by an increasingly retarded outwash from more distant areas. The results suggest a bimodal structural resistance to albumin movement: a relatively low resistance in the capillary membrane, and a considerable restriction to albumin transport located within the interstitial space.

KW - Animals

KW - Biomarkers

KW - Biopsy

KW - Capillary Permeability

KW - Capillary Resistance

KW - Ear

KW - Fibrinogen

KW - Iodine Radioisotopes

KW - Rabbits

KW - Serum Albumin

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 2051958

VL - 41

SP - 141

EP - 148

JO - Microvascular Research

JF - Microvascular Research

SN - 0026-2862

IS - 2

ER -

ID: 51664498