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The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner

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  1. Capsid-like particles decorated with the SARS-CoV-2 receptor-binding domain elicit strong virus neutralization activity

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  2. Common variants in Alzheimer's disease and risk stratification by polygenic risk scores

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  3. Risk variants and polygenic architecture of disruptive behavior disorders in the context of attention-deficit/hyperactivity disorder

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  4. Ultraviolet radiation drives mutations in a subset of mucosal melanomas

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  1. Capsid-like particles decorated with the SARS-CoV-2 receptor-binding domain elicit strong virus neutralization activity

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Risk Factors for Being Seronegative following SARS-CoV-2 Infection in a Large Cohort of Health Care Workers in Denmark

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  3. Labour market affiliation among non-bullied colleagues at work units with reported bullying

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  4. Risk of COVID-19 in health-care workers in Denmark: an observational cohort study

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Isolation of metastatic circulating tumor cells (CTCs) from cancer patients is of high value for disease monitoring and molecular characterization. Despite the development of many new CTC isolation platforms in the last decade, their isolation and detection has remained a challenge due to the lack of specific and sensitive markers. In this feasibility study, we present a method for CTC isolation based on the specific binding of the malaria rVAR2 protein to oncofetal chondroitin sulfate (ofCS). We show that rVAR2 efficiently captures CTCs from hepatic, lung, pancreatic, and prostate carcinoma patients with minimal contamination of peripheral blood mononuclear cells. Expression of ofCS is present on epithelial and mesenchymal cancer cells and is equally preserved during epithelial-mesenchymal transition of cancer cells. In 25 stage I-IV prostate cancer patient samples, CTC enumeration significantly correlates with disease stage. Lastly, rVAR2 targets a larger and more diverse population of CTCs compared to anti-EpCAM strategies.

Original languageEnglish
JournalNature Communications
Volume9
Issue number1
Pages (from-to)3279
ISSN2041-1723
DOIs
Publication statusPublished - 16 Aug 2018

    Research areas

  • Adaptation, Physiological, Antigens, Protozoan/metabolism, Cell Line, Tumor, Cell Separation, Epithelial Cell Adhesion Molecule/metabolism, Epithelial Cells/metabolism, Epithelial-Mesenchymal Transition, Humans, Leukocytes, Mononuclear/metabolism, Magnetics, Male, Mesoderm/metabolism, Microspheres, Neoplasm Staging, Neoplastic Cells, Circulating/metabolism, Pancreatic Neoplasms/blood, Prostatic Neoplasms/metabolism, Protein Binding, Proto-Oncogene Proteins p21(ras)/genetics, Recombinant Proteins/metabolism

ID: 56450083