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The VAR2CSA malaria protein efficiently retrieves circulating tumor cells in an EpCAM-independent manner

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  1. Whole genome landscapes of uveal melanoma show an ultraviolet radiation signature in iris tumours

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  2. Author Correction: Mutational and putative neoantigen load predict clinical benefit of adoptive T cell therapy in melanoma

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  3. Genomic analysis of male puberty timing highlights shared genetic basis with hair colour and lifespan

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  4. Functional hypoxia drives neuroplasticity and neurogenesis via brain erythropoietin

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  5. Combined burden and functional impact tests for cancer driver discovery using DriverPower

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  1. Capture and Detection of Circulating Glioma Cells Using the Recombinant VAR2CSA Malaria Protein

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  2. A proof-of-concept study for the design of a VLP-based combinatorial HPV and placental malaria vaccine

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Isolation of metastatic circulating tumor cells (CTCs) from cancer patients is of high value for disease monitoring and molecular characterization. Despite the development of many new CTC isolation platforms in the last decade, their isolation and detection has remained a challenge due to the lack of specific and sensitive markers. In this feasibility study, we present a method for CTC isolation based on the specific binding of the malaria rVAR2 protein to oncofetal chondroitin sulfate (ofCS). We show that rVAR2 efficiently captures CTCs from hepatic, lung, pancreatic, and prostate carcinoma patients with minimal contamination of peripheral blood mononuclear cells. Expression of ofCS is present on epithelial and mesenchymal cancer cells and is equally preserved during epithelial-mesenchymal transition of cancer cells. In 25 stage I-IV prostate cancer patient samples, CTC enumeration significantly correlates with disease stage. Lastly, rVAR2 targets a larger and more diverse population of CTCs compared to anti-EpCAM strategies.

Original languageEnglish
JournalNature Communications
Volume9
Issue number1
Pages (from-to)3279
ISSN2041-1723
DOIs
Publication statusPublished - 16 Aug 2018

    Research areas

  • Adaptation, Physiological, Antigens, Protozoan/metabolism, Cell Line, Tumor, Cell Separation, Epithelial Cell Adhesion Molecule/metabolism, Epithelial Cells/metabolism, Epithelial-Mesenchymal Transition, Humans, Leukocytes, Mononuclear/metabolism, Magnetics, Male, Mesoderm/metabolism, Microspheres, Neoplasm Staging, Neoplastic Cells, Circulating/metabolism, Pancreatic Neoplasms/blood, Prostatic Neoplasms/metabolism, Protein Binding, Proto-Oncogene Proteins p21(ras)/genetics, Recombinant Proteins/metabolism

ID: 56450083