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The structural basis for monoclonal antibody 5D2 binding to the tryptophan-rich loop of lipoprotein lipase

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  1. ANGPTL4: a new mode in the regulation of intravascular lipolysis

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  2. Expression and one-step purification of active LPL contemplated by biophysical considerations

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  3. The Urokinase Receptor (uPAR) as a "Trojan Horse" in Targeted Cancer Therapy: Challenges and Opportunities

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  • John Luz
  • Anne P Beigneux
  • DeeAnn K Asamoto
  • Cuiwen He
  • Wenxin Song
  • Christopher M Allan
  • Jazmin E Morales
  • Yiping Tu
  • Adam Kwok
  • Thomas Cottle
  • Muthuraman Meiyappan
  • Loren G Fong
  • Judy Kim
  • Michael Ploug
  • Stephen G Young
  • Gabriel Birrane
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For three decades, the lipoprotein lipase (LPL)-specific monoclonal antibody 5D2 has been used to investigate LPL structure/function and intravascular lipolysis. 5D2 has been used to measure LPL levels, block the triglyceride hydrolase activity of LPL, and prevent the propensity of concentrated LPL preparations to form homodimers. Two early studies on the location of the 5D2 epitope reached conflicting conclusions, but the more convincing report suggested that 5D2 binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The same loop had been implicated in lipoprotein binding. Using surface plasmon resonance, we showed that 5D2 binds with high affinity to a synthetic LPL peptide containing the Trp-rich loop of human (but not mouse) LPL. We also showed, by both fluorescence and ultraviolet resonance Raman spectroscopy, that the Trp-rich loop binds lipids. Finally, we used X-ray crystallography to solve the structure of the Trp-rich peptide bound to a 5D2 Fab fragment. The Trp-rich peptide contains a short alpha-helix, with two tryptophans projecting into the antigen recognition site. A proline substitution in the alpha-helix, found in mouse LPL, is expected to interfere with several hydrogen bonds, explaining why 5D2 cannot bind to mouse LPL.

Original languageEnglish
JournalJournal of Lipid Research
Volume61
Issue number10
Pages (from-to)1347-1359
ISSN0022-2275
DOIs
Publication statusPublished - 20 Jul 2020

    Research areas

  • X-ray crystallography, antibodies, lipid metabolism, protein structure, triglycerides

ID: 60626901