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The porcine corticospinal decussation: A combined neuronal tracing and tractography study

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Harvard

Bech, J, Glud, AN, Sangill, R, Petersen, M, Frandsen, J, Orlowski, D, West, MJ, Pedersen, M, Sørensen, JCH, Dyrby, TB & Bjarkam, CR 2018, 'The porcine corticospinal decussation: A combined neuronal tracing and tractography study' Brain Research Bulletin, vol. 142, pp. 253-262. https://doi.org/10.1016/j.brainresbull.2018.08.004

APA

Bech, J., Glud, A. N., Sangill, R., Petersen, M., Frandsen, J., Orlowski, D., ... Bjarkam, C. R. (2018). The porcine corticospinal decussation: A combined neuronal tracing and tractography study. Brain Research Bulletin, 142, 253-262. https://doi.org/10.1016/j.brainresbull.2018.08.004

CBE

Bech J, Glud AN, Sangill R, Petersen M, Frandsen J, Orlowski D, West MJ, Pedersen M, Sørensen JCH, Dyrby TB, Bjarkam CR. 2018. The porcine corticospinal decussation: A combined neuronal tracing and tractography study. Brain Research Bulletin. 142:253-262. https://doi.org/10.1016/j.brainresbull.2018.08.004

MLA

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Author

Bech, Johannes ; Glud, Andreas N ; Sangill, Ryan ; Petersen, Mikkel ; Frandsen, Jesper ; Orlowski, Dariusz ; West, Mark J ; Pedersen, Michael ; Sørensen, Jens Christian H ; Dyrby, Tim B ; Bjarkam, Carsten R. / The porcine corticospinal decussation: A combined neuronal tracing and tractography study. In: Brain Research Bulletin. 2018 ; Vol. 142. pp. 253-262.

Bibtex

@article{84ee23b40ffb4671890a15cffc7cd373,
title = "The porcine corticospinal decussation: A combined neuronal tracing and tractography study",
abstract = "BACKGROUND: Pigs and minipigs are increasingly used as non-primate large animal models for preclinical research on nervous system disorders resulting in motor dysfunction. Knowledge of the minipig pyramidal tract is therefore essential to support such models.AIM AND METHODS: This study used 5 female G{\"o}ttingen minipigs aging 11-15 months. The G{\"o}ttingen minipig corticospinal tract was investigated, in the same animals, with in vivo neuronal tracing and with postmortem diffusion weighted MRI tractography to provide a thorough insight in the encephalic distribution of this primary motor pathway and its decussation at the craniocervical junction.RESULTS: The two methods similarly outlined the course of the pyramidal tract from its origin in the motor cortex down through the internal capsule to the craniocervical junction, where both methods displayed an axonal crossover at the pyramid decussation. The degree of crossover was quantified with unbiased stereology, where 81-93{\%} of the traced corticospinal fibers crossed to the contralateral spinal cord. Accordingly, in the upper cervical spinal cord the corticospinal tract is primarily distributed in the contralateral lateral funiculus and in close relation to the gray matter, wherein some direct terminations on large ventral column gray matter neurons could be identified.DISCUSSION: The combination of neuronal tracing and tractography exploited the strengths of the respective methods to gain a better understanding of the encephalic distribution and craniocervical decussation of the G{\"o}ttingen minipig corticospinal tract. Moreover, a quantification of the crossing fibers was obtained from the tracing data, which was not possible with tractography. Our data indicate that the porcine corticospinal system is quite lateralized down to the investigated upper cervical levels. However, further elucidation of this point will require a full examination of the corticospinal tracing pattern into the caudal spinal cord combined with an analysis of the direct versus indirect termination pattern on the lower motor neurons.",
author = "Johannes Bech and Glud, {Andreas N} and Ryan Sangill and Mikkel Petersen and Jesper Frandsen and Dariusz Orlowski and West, {Mark J} and Michael Pedersen and S{\o}rensen, {Jens Christian H} and Dyrby, {Tim B} and Bjarkam, {Carsten R}",
note = "Copyright {\circledC} 2018. Published by Elsevier Inc.",
year = "2018",
month = "9",
day = "1",
doi = "10.1016/j.brainresbull.2018.08.004",
language = "English",
volume = "142",
pages = "253--262",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc",

}

RIS

TY - JOUR

T1 - The porcine corticospinal decussation: A combined neuronal tracing and tractography study

AU - Bech, Johannes

AU - Glud, Andreas N

AU - Sangill, Ryan

AU - Petersen, Mikkel

AU - Frandsen, Jesper

AU - Orlowski, Dariusz

AU - West, Mark J

AU - Pedersen, Michael

AU - Sørensen, Jens Christian H

AU - Dyrby, Tim B

AU - Bjarkam, Carsten R

N1 - Copyright © 2018. Published by Elsevier Inc.

PY - 2018/9/1

Y1 - 2018/9/1

N2 - BACKGROUND: Pigs and minipigs are increasingly used as non-primate large animal models for preclinical research on nervous system disorders resulting in motor dysfunction. Knowledge of the minipig pyramidal tract is therefore essential to support such models.AIM AND METHODS: This study used 5 female Göttingen minipigs aging 11-15 months. The Göttingen minipig corticospinal tract was investigated, in the same animals, with in vivo neuronal tracing and with postmortem diffusion weighted MRI tractography to provide a thorough insight in the encephalic distribution of this primary motor pathway and its decussation at the craniocervical junction.RESULTS: The two methods similarly outlined the course of the pyramidal tract from its origin in the motor cortex down through the internal capsule to the craniocervical junction, where both methods displayed an axonal crossover at the pyramid decussation. The degree of crossover was quantified with unbiased stereology, where 81-93% of the traced corticospinal fibers crossed to the contralateral spinal cord. Accordingly, in the upper cervical spinal cord the corticospinal tract is primarily distributed in the contralateral lateral funiculus and in close relation to the gray matter, wherein some direct terminations on large ventral column gray matter neurons could be identified.DISCUSSION: The combination of neuronal tracing and tractography exploited the strengths of the respective methods to gain a better understanding of the encephalic distribution and craniocervical decussation of the Göttingen minipig corticospinal tract. Moreover, a quantification of the crossing fibers was obtained from the tracing data, which was not possible with tractography. Our data indicate that the porcine corticospinal system is quite lateralized down to the investigated upper cervical levels. However, further elucidation of this point will require a full examination of the corticospinal tracing pattern into the caudal spinal cord combined with an analysis of the direct versus indirect termination pattern on the lower motor neurons.

AB - BACKGROUND: Pigs and minipigs are increasingly used as non-primate large animal models for preclinical research on nervous system disorders resulting in motor dysfunction. Knowledge of the minipig pyramidal tract is therefore essential to support such models.AIM AND METHODS: This study used 5 female Göttingen minipigs aging 11-15 months. The Göttingen minipig corticospinal tract was investigated, in the same animals, with in vivo neuronal tracing and with postmortem diffusion weighted MRI tractography to provide a thorough insight in the encephalic distribution of this primary motor pathway and its decussation at the craniocervical junction.RESULTS: The two methods similarly outlined the course of the pyramidal tract from its origin in the motor cortex down through the internal capsule to the craniocervical junction, where both methods displayed an axonal crossover at the pyramid decussation. The degree of crossover was quantified with unbiased stereology, where 81-93% of the traced corticospinal fibers crossed to the contralateral spinal cord. Accordingly, in the upper cervical spinal cord the corticospinal tract is primarily distributed in the contralateral lateral funiculus and in close relation to the gray matter, wherein some direct terminations on large ventral column gray matter neurons could be identified.DISCUSSION: The combination of neuronal tracing and tractography exploited the strengths of the respective methods to gain a better understanding of the encephalic distribution and craniocervical decussation of the Göttingen minipig corticospinal tract. Moreover, a quantification of the crossing fibers was obtained from the tracing data, which was not possible with tractography. Our data indicate that the porcine corticospinal system is quite lateralized down to the investigated upper cervical levels. However, further elucidation of this point will require a full examination of the corticospinal tracing pattern into the caudal spinal cord combined with an analysis of the direct versus indirect termination pattern on the lower motor neurons.

U2 - 10.1016/j.brainresbull.2018.08.004

DO - 10.1016/j.brainresbull.2018.08.004

M3 - Journal article

VL - 142

SP - 253

EP - 262

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

ER -

ID: 54922126