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The Capital Region of Denmark - a part of Copenhagen University Hospital
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The influence of sample collection methodology and sample preprocessing on the blood metabolic profile

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  • Benedicte Jørgenrud
  • Sirkku Jäntti
  • Ismo Mattila
  • Päivi Pöhö
  • Kjersti S Rønningen
  • Hannele Yki-Järvinen
  • Matej Orešič
  • Tuulia Hyötyläinen
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AIM: Blood serum and plasma have intrinsic differences in their composition and the preprocessing, such as clotting temperature in serum, and storage at room temperature may have further effect on metabolite concentrations.

METHODS: The influence of sampling preprocessing on the metabolic profiles in serum and different types of plasma was investigated using liquid chromatography and comprehensive 2D gas chromatography coupled to a mass spectrometer.

RESULTS: The profiles of polar metabolites were significantly dependent on the type of the sample, while lipid profiles were similar in serum and different types of plasma. Extended storage of plasma at room temperature resulted in degradation of lipids already after 1 day. Serum clotting at room temperature generally resulted in higher metabolite concentration compared with serum clotting on ice.

Original languageEnglish
JournalBioanalysis
Volume7
Issue number8
Pages (from-to)991-1006
Number of pages16
ISSN1757-6180
DOIs
Publication statusPublished - 2015
Externally publishedYes

    Research areas

  • Adult, Chromatography, Liquid/methods, Female, Gas Chromatography-Mass Spectrometry/methods, Humans, Lipids/analysis, Male, Metabolome, Metabolomics/methods, Middle Aged, Plasma/chemistry, Serum/chemistry, Specimen Handling/methods

ID: 54985767