Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

The influence of age and cardiorespiratory fitness on bioactive lipids in muscle

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Association Between Elevated suPAR, a New Biomarker of Inflammation, and Accelerated Aging

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Are Advances in Survival among the Oldest Old Seen across the Spectrum of Health and Functioning?

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Age- and Sex-Specific Changes in Lower-Limb Muscle Power Throughout the Lifespan

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. The Relationship between Cognitive Impairment and Upper Extremity Function in Older Primary Care Patients

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Effects of endogenous GIP in patients with type 2 diabetes

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. The training induced increase in whole-body peak fat oxidation rate may be attenuated with aging

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Mitochondrial dysfunction in adults after out-of-hospital cardiac arrest

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

Reduced insulin sensitivity is observed with aging and often explained by decreased physical activity. The mechanisms involved are not clarified, but bioactive lipids may play a role. We aimed to evaluate the influence of age and cardiorespiratory fitness on ceramide and diacylglycerol content in muscle and key proteins in lipid metabolism and insulin signaling.Healthy males were stratified by age into trained and untrained groups including 27 young (23.2±0.3 yrs.) and 33 aged (65.2±0.6 yrs.). Maximal oxygen uptake and body composition were measured and fasting blood samples and muscle biopsies obtained. Muscle ceramide and diacylglycerol were determined by thin-layer and gas-liquid chromatography and proteins by western blotting.We show that HOMA-IR was higher and VO2peak lower in aged compared to young. Total, saturated,C16:0 and C18:0 ceramide content were lower in muscle from aged compared to young. Intramuscular C18:1n9 and C20:4n6 content were higher in trained versus untrained. Content of total unsaturated and C16:1n7 diacylglycerol fatty acids were higher and C24:0 lower in muscle of aged versus young. Cardiorespiratory fitness had no impact on total diacylglycerol content. In conclusion, these data argue against intramuscular ceramide or diacylglycerol accumulation as driver of age-related insulin resistance in lean individuals.

Original languageEnglish
JournalThe journals of gerontology. Series A, Biological sciences and medical sciences
Volume74
Issue number6
Pages (from-to)778-786
ISSN1079-5006
DOIs
Publication statusPublished - May 2019

ID: 55467128