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The influence of age and aerobic fitness: effects on mitochondrial respiration in skeletal muscle

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Larsen, S ; Hey-Mogensen, Martin ; Rabøl, R ; Stride, N ; Helge, J W ; Dela, F. / The influence of age and aerobic fitness : effects on mitochondrial respiration in skeletal muscle. In: Acta physiologica (Oxford, England). 2012 ; Vol. 205, No. 3. pp. 423-32.

Bibtex

@article{5481d127504e4b7ea1639586978cf2f8,
title = "The influence of age and aerobic fitness: effects on mitochondrial respiration in skeletal muscle",
abstract = "AIM: Mitochondrial function has previously been studied in ageing, but never in humans matched for maximal oxygen uptake ((V)·O2max). Furthermore, the influence of ageing on mitochondrial substrate sensitivity is not known.METHODS: Skeletal muscle mitochondrial respiratory capacity and mitochondrial substrate sensitivity were measured by respirometry in young (23 ± 3 years) and middle-aged (53 ± 3 years) male subjects with similar (V)·O2max. Protocols for respirometry included titration of substrates for complex I (glutamate), complex II (succinate) and both (octanoyl carnitine) for calculation of substrate sensitivity (C(50) ). Myosin heavy chain (MHC) isoforms, citrate synthase (CS) and β-hydroxy-acyl-CoA-dehydrogenase (HAD) activity, mitochondrial DNA (mtDNA) content, protein levels of complexes I-V and antioxidant defence system [manganese superoxide dismutase (MnSOD)] were measured.RESULTS: No differences were found in maximal mitochondrial respiration or C(50) with glutamate (2.0 ± 0.3 and 1.8 ± 0.3 mm), succinate (3.7 ± 0.2 and 3.8 ± 0.4 mm) or octanoyl carnitine (47 ± 8 and 56 ± 7 μm) in young and middle-aged subjects respectively. Normalizing mitochondrial respiration to mtDNA young subjects had a higher (P < 0.05) respiratory capacity per mitochondrion compared to middle-aged subjects. HAD activity and mtDNA per mg tissue were higher in middle-aged compared to young subjects. Middle-aged had a higher MHC I isoform and a lower MHC IIX isoform content compared to young subjects.CONCLUSION: Mitochondrial substrate sensitivity is not affected by ageing. When young and middle-aged men are carefully matched for (V)·O2max, mitochondrial respiratory capacity is also similar. However, per mitochondrion respiratory capacity was lower in middle-aged compared to young subjects. Thus, when matched for (V)·O2max, middle-aged seem to require a higher mitochondrial content than young subjects.",
keywords = "3-Hydroxyacyl CoA Dehydrogenases, Adult, Aging, Biopsy, DNA, Mitochondrial, Electron Transport, Exercise, Humans, Male, Middle Aged, Mitochondria, Muscle, Muscle, Skeletal, Myosin Heavy Chains, Oxygen Consumption, Physical Fitness, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't",
author = "S Larsen and Martin Hey-Mogensen and R Rab{\o}l and N Stride and Helge, {J W} and F Dela",
note = "{\textcopyright} 2012 The Authors Acta Physiologica {\textcopyright} 2012 Scandinavian Physiological Society.",
year = "2012",
month = jul,
doi = "10.1111/j.1748-1716.2012.02408.x",
language = "English",
volume = "205",
pages = "423--32",
journal = "Acta Physiologica",
issn = "1748-1708",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - The influence of age and aerobic fitness

T2 - effects on mitochondrial respiration in skeletal muscle

AU - Larsen, S

AU - Hey-Mogensen, Martin

AU - Rabøl, R

AU - Stride, N

AU - Helge, J W

AU - Dela, F

N1 - © 2012 The Authors Acta Physiologica © 2012 Scandinavian Physiological Society.

PY - 2012/7

Y1 - 2012/7

N2 - AIM: Mitochondrial function has previously been studied in ageing, but never in humans matched for maximal oxygen uptake ((V)·O2max). Furthermore, the influence of ageing on mitochondrial substrate sensitivity is not known.METHODS: Skeletal muscle mitochondrial respiratory capacity and mitochondrial substrate sensitivity were measured by respirometry in young (23 ± 3 years) and middle-aged (53 ± 3 years) male subjects with similar (V)·O2max. Protocols for respirometry included titration of substrates for complex I (glutamate), complex II (succinate) and both (octanoyl carnitine) for calculation of substrate sensitivity (C(50) ). Myosin heavy chain (MHC) isoforms, citrate synthase (CS) and β-hydroxy-acyl-CoA-dehydrogenase (HAD) activity, mitochondrial DNA (mtDNA) content, protein levels of complexes I-V and antioxidant defence system [manganese superoxide dismutase (MnSOD)] were measured.RESULTS: No differences were found in maximal mitochondrial respiration or C(50) with glutamate (2.0 ± 0.3 and 1.8 ± 0.3 mm), succinate (3.7 ± 0.2 and 3.8 ± 0.4 mm) or octanoyl carnitine (47 ± 8 and 56 ± 7 μm) in young and middle-aged subjects respectively. Normalizing mitochondrial respiration to mtDNA young subjects had a higher (P < 0.05) respiratory capacity per mitochondrion compared to middle-aged subjects. HAD activity and mtDNA per mg tissue were higher in middle-aged compared to young subjects. Middle-aged had a higher MHC I isoform and a lower MHC IIX isoform content compared to young subjects.CONCLUSION: Mitochondrial substrate sensitivity is not affected by ageing. When young and middle-aged men are carefully matched for (V)·O2max, mitochondrial respiratory capacity is also similar. However, per mitochondrion respiratory capacity was lower in middle-aged compared to young subjects. Thus, when matched for (V)·O2max, middle-aged seem to require a higher mitochondrial content than young subjects.

AB - AIM: Mitochondrial function has previously been studied in ageing, but never in humans matched for maximal oxygen uptake ((V)·O2max). Furthermore, the influence of ageing on mitochondrial substrate sensitivity is not known.METHODS: Skeletal muscle mitochondrial respiratory capacity and mitochondrial substrate sensitivity were measured by respirometry in young (23 ± 3 years) and middle-aged (53 ± 3 years) male subjects with similar (V)·O2max. Protocols for respirometry included titration of substrates for complex I (glutamate), complex II (succinate) and both (octanoyl carnitine) for calculation of substrate sensitivity (C(50) ). Myosin heavy chain (MHC) isoforms, citrate synthase (CS) and β-hydroxy-acyl-CoA-dehydrogenase (HAD) activity, mitochondrial DNA (mtDNA) content, protein levels of complexes I-V and antioxidant defence system [manganese superoxide dismutase (MnSOD)] were measured.RESULTS: No differences were found in maximal mitochondrial respiration or C(50) with glutamate (2.0 ± 0.3 and 1.8 ± 0.3 mm), succinate (3.7 ± 0.2 and 3.8 ± 0.4 mm) or octanoyl carnitine (47 ± 8 and 56 ± 7 μm) in young and middle-aged subjects respectively. Normalizing mitochondrial respiration to mtDNA young subjects had a higher (P < 0.05) respiratory capacity per mitochondrion compared to middle-aged subjects. HAD activity and mtDNA per mg tissue were higher in middle-aged compared to young subjects. Middle-aged had a higher MHC I isoform and a lower MHC IIX isoform content compared to young subjects.CONCLUSION: Mitochondrial substrate sensitivity is not affected by ageing. When young and middle-aged men are carefully matched for (V)·O2max, mitochondrial respiratory capacity is also similar. However, per mitochondrion respiratory capacity was lower in middle-aged compared to young subjects. Thus, when matched for (V)·O2max, middle-aged seem to require a higher mitochondrial content than young subjects.

KW - 3-Hydroxyacyl CoA Dehydrogenases

KW - Adult

KW - Aging

KW - Biopsy

KW - DNA, Mitochondrial

KW - Electron Transport

KW - Exercise

KW - Humans

KW - Male

KW - Middle Aged

KW - Mitochondria, Muscle

KW - Muscle, Skeletal

KW - Myosin Heavy Chains

KW - Oxygen Consumption

KW - Physical Fitness

KW - Comparative Study

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1111/j.1748-1716.2012.02408.x

DO - 10.1111/j.1748-1716.2012.02408.x

M3 - Journal article

C2 - 22212519

VL - 205

SP - 423

EP - 432

JO - Acta Physiologica

JF - Acta Physiologica

SN - 1748-1708

IS - 3

ER -

ID: 51616000