The human superior cervical ganglion: neuropeptides and peptide receptors

J Tajti; S Möller; R Uddman; I Bodi; L Edvinsson

The human superior cervical ganglion: neuropeptides and peptide receptors

J Tajti, S Möller, R Uddman, I Bodi, L Edvinsson

36 Citations (Scopus)

Abstract

Noradrenaline (NA)- and neuropeptide Y (NPY)-containing cell bodies were found to occur in high numbers (>75% of all cells were positive) in the human superior cervical ganglion and distributed homogeneously throughout the ganglion and showed colocalisation. A few cell bodies were VIP-immunoreactive (-ir) (less than 5%) but none of them showed NOS-, CGRP- or SP-ir. Receptor mRNA expression was studied with RT-PCR. Total RNA from the superior cervical ganglion was successfully extracted. By using appropriate sense and antisense oligonucleotides designed from the published human sequences, we could show the presence of mRNA for the human NPY Y1, NPY Y2 and VPAC1 receptors but not CGRP1 receptor mRNA.

Original language	English
Journal	Neuroscience Letters
Volume	263
Issue number	2-3
Pages (from-to)	121-4
Number of pages	4
ISSN	0304-3940
Publication status	Published - 26 Mar 1999
Externally published	Yes

Keywords

Aged
Aged, 80 and over
Female
Humans
Immunohistochemistry
Male
Middle Aged
Neuropeptides
RNA, Messenger
Receptors, Calcitonin Gene-Related Peptide
Receptors, Neuropeptide
Receptors, Neuropeptide Y
Reverse Transcriptase Polymerase Chain Reaction
Superior Cervical Ganglion
Transcription, Genetic
Vasoactive Intestinal Peptide

Cite this

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title = "The human superior cervical ganglion: neuropeptides and peptide receptors",

abstract = "Noradrenaline (NA)- and neuropeptide Y (NPY)-containing cell bodies were found to occur in high numbers (>75\% of all cells were positive) in the human superior cervical ganglion and distributed homogeneously throughout the ganglion and showed colocalisation. A few cell bodies were VIP-immunoreactive (-ir) (less than 5\%) but none of them showed NOS-, CGRP- or SP-ir. Receptor mRNA expression was studied with RT-PCR. Total RNA from the superior cervical ganglion was successfully extracted. By using appropriate sense and antisense oligonucleotides designed from the published human sequences, we could show the presence of mRNA for the human NPY Y1, NPY Y2 and VPAC1 receptors but not CGRP1 receptor mRNA.",

keywords = "Aged, Aged, 80 and over, Female, Humans, Immunohistochemistry, Male, Middle Aged, Neuropeptides, RNA, Messenger, Receptors, Calcitonin Gene-Related Peptide, Receptors, Neuropeptide, Receptors, Neuropeptide Y, Reverse Transcriptase Polymerase Chain Reaction, Superior Cervical Ganglion, Transcription, Genetic, Vasoactive Intestinal Peptide",

author = "J Tajti and S M{\"o}ller and R Uddman and I Bodi and L Edvinsson",

year = "1999",

month = mar,

day = "26",

language = "English",

volume = "263",

pages = "121--4",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "2-3",

}

TY - JOUR

T1 - The human superior cervical ganglion

T2 - neuropeptides and peptide receptors

AU - Tajti, J

AU - Möller, S

AU - Uddman, R

AU - Bodi, I

AU - Edvinsson, L

PY - 1999/3/26

Y1 - 1999/3/26

N2 - Noradrenaline (NA)- and neuropeptide Y (NPY)-containing cell bodies were found to occur in high numbers (>75% of all cells were positive) in the human superior cervical ganglion and distributed homogeneously throughout the ganglion and showed colocalisation. A few cell bodies were VIP-immunoreactive (-ir) (less than 5%) but none of them showed NOS-, CGRP- or SP-ir. Receptor mRNA expression was studied with RT-PCR. Total RNA from the superior cervical ganglion was successfully extracted. By using appropriate sense and antisense oligonucleotides designed from the published human sequences, we could show the presence of mRNA for the human NPY Y1, NPY Y2 and VPAC1 receptors but not CGRP1 receptor mRNA.

AB - Noradrenaline (NA)- and neuropeptide Y (NPY)-containing cell bodies were found to occur in high numbers (>75% of all cells were positive) in the human superior cervical ganglion and distributed homogeneously throughout the ganglion and showed colocalisation. A few cell bodies were VIP-immunoreactive (-ir) (less than 5%) but none of them showed NOS-, CGRP- or SP-ir. Receptor mRNA expression was studied with RT-PCR. Total RNA from the superior cervical ganglion was successfully extracted. By using appropriate sense and antisense oligonucleotides designed from the published human sequences, we could show the presence of mRNA for the human NPY Y1, NPY Y2 and VPAC1 receptors but not CGRP1 receptor mRNA.

KW - Aged

KW - Aged, 80 and over

KW - Female

KW - Humans

KW - Immunohistochemistry

KW - Male

KW - Middle Aged

KW - Neuropeptides

KW - RNA, Messenger

KW - Receptors, Calcitonin Gene-Related Peptide

KW - Receptors, Neuropeptide

KW - Receptors, Neuropeptide Y

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Superior Cervical Ganglion

KW - Transcription, Genetic

KW - Vasoactive Intestinal Peptide

UR - https://www.scopus.com/pages/publications/0345435253

M3 - Journal article

C2 - 10213150

SN - 0304-3940

VL - 263

SP - 121

EP - 124

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2-3

ER -

The human superior cervical ganglion: neuropeptides and peptide receptors

Abstract

Keywords

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