The human placenta from heavy smokers: evaluation of vasoactive peptides by immunohistochemistry.

H V Clausen, L Grupe Larsen, A Jørgensen, M Bzorek

4 Citations (Scopus)


The study aimed to demonstrate the expression of nitric oxide converting enzyme, nitric oxide synthase (e-NOS), and endothelin-1 (Et-1) in formalin-fixed paraffin-embedded placental tissue, and to demonstrate a difference in staining intensity between heavy smokers and non-smokers. Term placentas from pregnancies from otherwise healthy women smoking 15 or more cigarettes per day (heavy smokers) and term placentas from a matching group of non-smokers were included. The antibodies for Et-1 and e-NOS are recommended for cryostat sections. We evaluated the antibodies on paraffin-embedded tissue combined with the streptavidin-biotin-peroxidase technique. Et-1 and e-NOS were demonstrated in the placental vasculature, the trophoblast, and the amnion. A blinded comparative study showed no reproducible significant differences in the staining intensity of the antigen-antibody reaction to Et-1 and e-NOS between the two groups.
Udgivelsesdato: 2007-Jan
Original languageEnglish
JournalAPMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Issue number1
Pages (from-to)22-9
Number of pages7
Publication statusPublished - 2007


  • Adult
  • Endothelin-1
  • Endothelium, Vascular
  • Female
  • Humans
  • Immunohistochemistry
  • Nitric Oxide Synthase
  • Placenta
  • Pregnancy
  • Pregnancy Complications
  • Smoking

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