The effect of growth hormone on the development of diabetic kidney disease in rats

Daniel Landau, Eytan Israel, Inessa Rivkis, Leonid Kachko, Bieke F Schrijvers, Allan Flyvbjerg, Moshe Phillip, Yael Segev

29 Citations (Scopus)

Abstract

BACKGROUND: Nephropathy is the most severe complication of diabetes mellitus. We investigated the effect of exogenous growth hormone (GH) administration on renal function and matrix deposition in the streptozotocin (STZ) model of type I-diabetic rat.

METHODS: Adult female STZ-diabetic rats (D), non-diabetic control rats injected with saline (C) and control and diabetic rats injected with bovine GH for 3 months (CGH and DGH, respectively) were used.

RESULTS: The usual renal hypertrophy seen in D animals was more pronounced in the DGH group. Creatinine clearance increased only in the D rats, but not in the other groups, including DGH. Albuminuria was observed in the D animals but was significantly elevated in the DGH group. Glomeruli from DGH animals showed more extensive matrix accumulation (manifested as an increase in mesangial/glomerular area ratio). Renal extractable insulin-like growth factor (IGF-I) mRNA was decreased in the D and DGH groups, but renal IGF-I protein was not significantly increased. Renal IGF binding protein-1 was increased in the D groups and further increased in the DGH group, at both the mRNA and protein levels.

CONCLUSIONS: GH-treated diabetic rats had less hyperfiltration and more albuminuria, concomitant with more glomerular matrix deposition, when compared with regular diabetic animals. This was associated with a significant increase in renal IGFBP-1, and dissociated from IGF-I changes. Thus, in this model, GH exacerbates the course of diabetic kidney disease.

Original languageEnglish
JournalNephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
Volume18
Issue number4
Pages (from-to)694-702
Number of pages9
ISSN0931-0509
Publication statusPublished - Apr 2003
Externally publishedYes

Keywords

  • Albuminuria
  • Analysis of Variance
  • Animals
  • Base Sequence
  • Biopsy, Needle
  • Blotting, Western
  • Diabetes Mellitus, Experimental
  • Diabetic Nephropathies
  • Disease Models, Animal
  • Growth Hormone
  • Immunohistochemistry
  • Kidney
  • Kidney Function Tests
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Probability
  • RNA, Messenger
  • Rats
  • Rats, Wistar
  • Receptor, IGF Type 1
  • Reference Values
  • Statistics, Nonparametric
  • Streptozocin
  • Urinalysis
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't

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