The effect of adenovirus-mediated gene expression of FHIT in small cell lung cancer cells

Roza Zandi; Kai Xu; Hans S Poulsen; Jack A Roth; Lin Ji

doi:10.3109/07357907.2011.626475

The effect of adenovirus-mediated gene expression of FHIT in small cell lung cancer cells

Roza Zandi, Kai Xu, Hans S Poulsen, Jack A Roth, Lin Ji

7 Citations (Scopus)

Abstract

The candidate tumor suppressor fragile histidine traid (FHIT) is frequently inactivated in small cell lung cancer (SCLC). Mutations in the p53 gene also occur in the majority of SCLC leading to the accumulation of the mutant protein. Here we evaluated the effect of FHIT gene therapy alone or in combination with the mutant p53-reactivating molecule, PRIMA-1(Met)/APR-246, in SCLC. Overexpression of FHIT by recombinant adenoviral vector (Ad-FHIT)-mediated gene transfer in SCLC cells inhibited their growth by inducing apoptosis and when combined with PRIMA-1(Met)/APR-246, a synergistic cell growth inhibition was achieved.

Original language	English
Journal	Cancer Investigation
Volume	29
Issue number	10
Pages (from-to)	683-91
Number of pages	9
ISSN	0735-7907
DOIs	https://doi.org/10.3109/07357907.2011.626475
Publication status	Published - 2011

Keywords

Acid Anhydride Hydrolases
Adenoviridae
Apoptosis
Carcinoma, Small Cell
Cell Line, Tumor
Gene Expression
Gene Therapy
Humans
Lung Neoplasms
Neoplasm Proteins
RNA, Messenger
Tumor Suppressor Protein p53

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10.3109/07357907.2011.626475

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title = "The effect of adenovirus-mediated gene expression of FHIT in small cell lung cancer cells",

abstract = "The candidate tumor suppressor fragile histidine traid (FHIT) is frequently inactivated in small cell lung cancer (SCLC). Mutations in the p53 gene also occur in the majority of SCLC leading to the accumulation of the mutant protein. Here we evaluated the effect of FHIT gene therapy alone or in combination with the mutant p53-reactivating molecule, PRIMA-1(Met)/APR-246, in SCLC. Overexpression of FHIT by recombinant adenoviral vector (Ad-FHIT)-mediated gene transfer in SCLC cells inhibited their growth by inducing apoptosis and when combined with PRIMA-1(Met)/APR-246, a synergistic cell growth inhibition was achieved.",

keywords = "Acid Anhydride Hydrolases, Adenoviridae, Apoptosis, Carcinoma, Small Cell, Cell Line, Tumor, Gene Expression, Gene Therapy, Humans, Lung Neoplasms, Neoplasm Proteins, RNA, Messenger, Tumor Suppressor Protein p53",

author = "Roza Zandi and Kai Xu and Poulsen, \{Hans S\} and Roth, \{Jack A\} and Lin Ji",

year = "2011",

doi = "10.3109/07357907.2011.626475",

language = "English",

volume = "29",

pages = "683--91",

journal = "Cancer Investigation",

issn = "0735-7907",

publisher = "Taylor \& Francis Group LLC Philadelphia",

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TY - JOUR

T1 - The effect of adenovirus-mediated gene expression of FHIT in small cell lung cancer cells

AU - Zandi, Roza

AU - Xu, Kai

AU - Poulsen, Hans S

AU - Roth, Jack A

AU - Ji, Lin

PY - 2011

Y1 - 2011

N2 - The candidate tumor suppressor fragile histidine traid (FHIT) is frequently inactivated in small cell lung cancer (SCLC). Mutations in the p53 gene also occur in the majority of SCLC leading to the accumulation of the mutant protein. Here we evaluated the effect of FHIT gene therapy alone or in combination with the mutant p53-reactivating molecule, PRIMA-1(Met)/APR-246, in SCLC. Overexpression of FHIT by recombinant adenoviral vector (Ad-FHIT)-mediated gene transfer in SCLC cells inhibited their growth by inducing apoptosis and when combined with PRIMA-1(Met)/APR-246, a synergistic cell growth inhibition was achieved.

AB - The candidate tumor suppressor fragile histidine traid (FHIT) is frequently inactivated in small cell lung cancer (SCLC). Mutations in the p53 gene also occur in the majority of SCLC leading to the accumulation of the mutant protein. Here we evaluated the effect of FHIT gene therapy alone or in combination with the mutant p53-reactivating molecule, PRIMA-1(Met)/APR-246, in SCLC. Overexpression of FHIT by recombinant adenoviral vector (Ad-FHIT)-mediated gene transfer in SCLC cells inhibited their growth by inducing apoptosis and when combined with PRIMA-1(Met)/APR-246, a synergistic cell growth inhibition was achieved.

KW - Acid Anhydride Hydrolases

KW - Adenoviridae

KW - Apoptosis

KW - Carcinoma, Small Cell

KW - Cell Line, Tumor

KW - Gene Expression

KW - Gene Therapy

KW - Humans

KW - Lung Neoplasms

KW - Neoplasm Proteins

KW - RNA, Messenger

KW - Tumor Suppressor Protein p53

UR - https://www.scopus.com/pages/publications/81255203813

U2 - 10.3109/07357907.2011.626475

DO - 10.3109/07357907.2011.626475

M3 - Journal article

C2 - 22085272

SN - 0735-7907

VL - 29

SP - 683

EP - 691

JO - Cancer Investigation

JF - Cancer Investigation

IS - 10

ER -

The effect of adenovirus-mediated gene expression of FHIT in small cell lung cancer cells

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Keywords

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