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The cGMP-Degrading Enzyme Phosphodiesterase-5 (PDE5) in Cerebral Small Arteries of Older People

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Vasita, Ekta ; Yasmeen, Saiqa ; Andoh, Joycelyn ; Bridges, Leslie R ; Kruuse, Christina ; Pauls, Mathilde M H ; Pereira, Anthony C ; Hainsworth, Atticus H. / The cGMP-Degrading Enzyme Phosphodiesterase-5 (PDE5) in Cerebral Small Arteries of Older People. In: Journal of Neuropathology and Experimental Neurology. 2019 ; Vol. 78, No. 2. pp. 191-194.

Bibtex

@article{6c3eb4091907403687dc2547aa47df04,
title = "The cGMP-Degrading Enzyme Phosphodiesterase-5 (PDE5) in Cerebral Small Arteries of Older People",
abstract = "Cerebral small vessel disease in deep penetrating arteries is a major cause of lacunar infarcts, white matter lesions and vascular cognitive impairment. Local cerebral blood flow in these small vessels is controlled by endothelial-derived nitric oxide, which exerts a primary vasodilator stimulus on vascular myocytes, via cytoplasmic cyclic GMP. Here, we investigated whether the cGMP-degrading enzyme phosphodiesterase-5 (PDE5) is present in small penetrating arteries in the deep subcortical white matter of older people. Frontal cortical tissue blocks were examined from donated brains of older people (n = 42, 24 male: 18 female, median age 81, range: 59-100 years). PDE5, detected by immunohistochemical labeling, was graded as absent, sparse, or abundant in vascular cells within small arteries in subcortical white matter (vessel outer diameter: 20-100 µm). PDE5 labeling within arterial myocytes was detected in all cases. Degree of PDE5 expression (absent, sparse, or abundant) was not associated with age or with neuropathological diagnosis of small vessel disease. In conclusion, PDE5 is present in vascular myocytes within small penetrating arteries in older people. This is a potential molecular target for pharmacological interventions.",
keywords = "Aged, Aged, 80 and over, Brain/enzymology, Cerebral Arteries/enzymology, Cyclic Nucleotide Phosphodiesterases, Type 5/metabolism, Female, Humans, Male, Middle Aged, Muscle Cells/enzymology, Muscle, Smooth, Vascular/enzymology, White Matter/enzymology",
author = "Ekta Vasita and Saiqa Yasmeen and Joycelyn Andoh and Bridges, {Leslie R} and Christina Kruuse and Pauls, {Mathilde M H} and Pereira, {Anthony C} and Hainsworth, {Atticus H}",
year = "2019",
month = feb,
day = "1",
doi = "10.1093/jnen/nly117",
language = "English",
volume = "78",
pages = "191--194",
journal = "Journal of Psychotherapy Practice and Research",
issn = "0002-9564",
publisher = "Lippincott Williams & Wilkins",
number = "2",

}

RIS

TY - JOUR

T1 - The cGMP-Degrading Enzyme Phosphodiesterase-5 (PDE5) in Cerebral Small Arteries of Older People

AU - Vasita, Ekta

AU - Yasmeen, Saiqa

AU - Andoh, Joycelyn

AU - Bridges, Leslie R

AU - Kruuse, Christina

AU - Pauls, Mathilde M H

AU - Pereira, Anthony C

AU - Hainsworth, Atticus H

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Cerebral small vessel disease in deep penetrating arteries is a major cause of lacunar infarcts, white matter lesions and vascular cognitive impairment. Local cerebral blood flow in these small vessels is controlled by endothelial-derived nitric oxide, which exerts a primary vasodilator stimulus on vascular myocytes, via cytoplasmic cyclic GMP. Here, we investigated whether the cGMP-degrading enzyme phosphodiesterase-5 (PDE5) is present in small penetrating arteries in the deep subcortical white matter of older people. Frontal cortical tissue blocks were examined from donated brains of older people (n = 42, 24 male: 18 female, median age 81, range: 59-100 years). PDE5, detected by immunohistochemical labeling, was graded as absent, sparse, or abundant in vascular cells within small arteries in subcortical white matter (vessel outer diameter: 20-100 µm). PDE5 labeling within arterial myocytes was detected in all cases. Degree of PDE5 expression (absent, sparse, or abundant) was not associated with age or with neuropathological diagnosis of small vessel disease. In conclusion, PDE5 is present in vascular myocytes within small penetrating arteries in older people. This is a potential molecular target for pharmacological interventions.

AB - Cerebral small vessel disease in deep penetrating arteries is a major cause of lacunar infarcts, white matter lesions and vascular cognitive impairment. Local cerebral blood flow in these small vessels is controlled by endothelial-derived nitric oxide, which exerts a primary vasodilator stimulus on vascular myocytes, via cytoplasmic cyclic GMP. Here, we investigated whether the cGMP-degrading enzyme phosphodiesterase-5 (PDE5) is present in small penetrating arteries in the deep subcortical white matter of older people. Frontal cortical tissue blocks were examined from donated brains of older people (n = 42, 24 male: 18 female, median age 81, range: 59-100 years). PDE5, detected by immunohistochemical labeling, was graded as absent, sparse, or abundant in vascular cells within small arteries in subcortical white matter (vessel outer diameter: 20-100 µm). PDE5 labeling within arterial myocytes was detected in all cases. Degree of PDE5 expression (absent, sparse, or abundant) was not associated with age or with neuropathological diagnosis of small vessel disease. In conclusion, PDE5 is present in vascular myocytes within small penetrating arteries in older people. This is a potential molecular target for pharmacological interventions.

KW - Aged

KW - Aged, 80 and over

KW - Brain/enzymology

KW - Cerebral Arteries/enzymology

KW - Cyclic Nucleotide Phosphodiesterases, Type 5/metabolism

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Muscle Cells/enzymology

KW - Muscle, Smooth, Vascular/enzymology

KW - White Matter/enzymology

U2 - 10.1093/jnen/nly117

DO - 10.1093/jnen/nly117

M3 - Journal article

C2 - 30590671

VL - 78

SP - 191

EP - 194

JO - Journal of Psychotherapy Practice and Research

JF - Journal of Psychotherapy Practice and Research

SN - 0002-9564

IS - 2

ER -

ID: 59116042