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Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads

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Mamsen, Linn S ; Ernst, Emil H ; Borup, Rehannah ; Larsen, Agnete ; Olesen, Rasmus H ; Ernst, Erik ; Anderson, Richard A ; Kristensen, Stine G ; Andersen, Claus Y. / Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads. In: Scientific Reports. 2017 ; Vol. 7, No. 1. pp. 15961.

Bibtex

@article{898fa6b2583247b59b3338383eccb2ab,
title = "Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads",
abstract = "The precise timing and sequence of changes in expression of key genes and proteins during human sex-differentiation and onset of steroidogenesis was evaluated by whole-genome expression in 67 first trimester human embryonic and fetal ovaries and testis and confirmed by qPCR and immunohistochemistry (IHC). SRY/SOX9 expression initiated in testis around day 40 pc, followed by initiation of AMH and steroidogenic genes required for androgen production at day 53 pc. In ovaries, gene expression of RSPO1, LIN28, FOXL2, WNT2B, and ETV5, were significantly higher than in testis, whereas GLI1 was significantly higher in testis than ovaries. Gene expression was confirmed by IHC for GAGE, SOX9, AMH, CYP17A1, LIN28, WNT2B, ETV5 and GLI1. Gene expression was not associated with the maternal smoking habits. Collectively, a precise temporal determination of changes in expression of key genes involved in human sex-differentiation is defined, with identification of new genes of potential importance.",
keywords = "Journal Article",
author = "Mamsen, {Linn S} and Ernst, {Emil H} and Rehannah Borup and Agnete Larsen and Olesen, {Rasmus H} and Erik Ernst and Anderson, {Richard A} and Kristensen, {Stine G} and Andersen, {Claus Y}",
year = "2017",
month = "11",
day = "21",
doi = "10.1038/s41598-017-15931-3",
language = "English",
volume = "7",
pages = "15961",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Temporal expression pattern of genes during the period of sex differentiation in human embryonic gonads

AU - Mamsen, Linn S

AU - Ernst, Emil H

AU - Borup, Rehannah

AU - Larsen, Agnete

AU - Olesen, Rasmus H

AU - Ernst, Erik

AU - Anderson, Richard A

AU - Kristensen, Stine G

AU - Andersen, Claus Y

PY - 2017/11/21

Y1 - 2017/11/21

N2 - The precise timing and sequence of changes in expression of key genes and proteins during human sex-differentiation and onset of steroidogenesis was evaluated by whole-genome expression in 67 first trimester human embryonic and fetal ovaries and testis and confirmed by qPCR and immunohistochemistry (IHC). SRY/SOX9 expression initiated in testis around day 40 pc, followed by initiation of AMH and steroidogenic genes required for androgen production at day 53 pc. In ovaries, gene expression of RSPO1, LIN28, FOXL2, WNT2B, and ETV5, were significantly higher than in testis, whereas GLI1 was significantly higher in testis than ovaries. Gene expression was confirmed by IHC for GAGE, SOX9, AMH, CYP17A1, LIN28, WNT2B, ETV5 and GLI1. Gene expression was not associated with the maternal smoking habits. Collectively, a precise temporal determination of changes in expression of key genes involved in human sex-differentiation is defined, with identification of new genes of potential importance.

AB - The precise timing and sequence of changes in expression of key genes and proteins during human sex-differentiation and onset of steroidogenesis was evaluated by whole-genome expression in 67 first trimester human embryonic and fetal ovaries and testis and confirmed by qPCR and immunohistochemistry (IHC). SRY/SOX9 expression initiated in testis around day 40 pc, followed by initiation of AMH and steroidogenic genes required for androgen production at day 53 pc. In ovaries, gene expression of RSPO1, LIN28, FOXL2, WNT2B, and ETV5, were significantly higher than in testis, whereas GLI1 was significantly higher in testis than ovaries. Gene expression was confirmed by IHC for GAGE, SOX9, AMH, CYP17A1, LIN28, WNT2B, ETV5 and GLI1. Gene expression was not associated with the maternal smoking habits. Collectively, a precise temporal determination of changes in expression of key genes involved in human sex-differentiation is defined, with identification of new genes of potential importance.

KW - Journal Article

U2 - 10.1038/s41598-017-15931-3

DO - 10.1038/s41598-017-15931-3

M3 - Journal article

VL - 7

SP - 15961

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

ER -

ID: 52193380