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Technical advance: immunophenotypical characterization of human neutrophil differentiation

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@article{fcf302d9aa6b42098a3f709a64743917,
title = "Technical advance: immunophenotypical characterization of human neutrophil differentiation",
abstract = "The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.",
keywords = "Blotting, Western, Bone Marrow, Cell Differentiation, Cell Line, Cell Separation, Flow Cytometry, Granulocyte Precursor Cells, Granulocytes, Humans, Immunoenzyme Techniques, Immunophenotyping, Myeloid Cells, Myeloid Progenitor Cells, Neutrophils, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction",
author = "Mora-Jensen, {Helena Isabel} and Johan Jendholm and Anna Fossum and Porse, {Bo T} and Niels Borregaard and Kim Theilgaard-M{\"o}nch",
year = "2011",
doi = "10.1189/jlb.0311123",
language = "English",
volume = "90",
pages = "629--34",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "Federation of American Societies for Experimental Biology",
number = "3",

}

RIS

TY - JOUR

T1 - Technical advance: immunophenotypical characterization of human neutrophil differentiation

AU - Mora-Jensen, Helena Isabel

AU - Jendholm, Johan

AU - Fossum, Anna

AU - Porse, Bo T

AU - Borregaard, Niels

AU - Theilgaard-Mönch, Kim

PY - 2011

Y1 - 2011

N2 - The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

AB - The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

KW - Blotting, Western

KW - Bone Marrow

KW - Cell Differentiation

KW - Cell Line

KW - Cell Separation

KW - Flow Cytometry

KW - Granulocyte Precursor Cells

KW - Granulocytes

KW - Humans

KW - Immunoenzyme Techniques

KW - Immunophenotyping

KW - Myeloid Cells

KW - Myeloid Progenitor Cells

KW - Neutrophils

KW - RNA, Messenger

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.1189/jlb.0311123

DO - 10.1189/jlb.0311123

M3 - Journal article

VL - 90

SP - 629

EP - 634

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 3

ER -

ID: 33232466