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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Technical advance: immunophenotypical characterization of human neutrophil differentiation

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  1. Niels Borregaard, M.D. (1951-2017)

    Research output: Contribution to journalJournal articleCommunication

  2. Smoking reduces circulating CD26hiCD161hi MAIT cells in healthy individuals and patients with multiple sclerosis

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  3. CD4+/CD8+ double-positive T cells: more than just a developmental stage?

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  4. Molecular and stimulus-response profiles illustrate heterogeneity between peripheral and cord blood-derived human mast cells

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  1. Mutant CEBPA directly drives the expression of the targetable tumor-promoting factor CD73 in AML

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  2. Heterozygous loss of Srp72 in mice is not associated with major hematological phenotypes

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  3. A programmed wave of uridylation-primed mRNA degradation is essential for meiotic progression and mammalian spermatogenesis

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  4. The splicing factor RBM25 controls MYC activity in acute myeloid leukemia

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  5. Leukemogenic nucleophosmin mutation disrupts the transcription factor hub regulating granulo-monocytic fates

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The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.
Original languageEnglish
JournalJournal of Leukocyte Biology
Volume90
Issue number3
Pages (from-to)629-34
Number of pages6
ISSN0741-5400
DOIs
Publication statusPublished - 2011

    Research areas

  • Blotting, Western, Bone Marrow, Cell Differentiation, Cell Line, Cell Separation, Flow Cytometry, Granulocyte Precursor Cells, Granulocytes, Humans, Immunoenzyme Techniques, Immunophenotyping, Myeloid Cells, Myeloid Progenitor Cells, Neutrophils, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction

ID: 33232466