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The Capital Region of Denmark - a part of Copenhagen University Hospital
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Supersensitive gastrin assay using antibodies raised against a cholecystokinin homolog

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  1. Circadian variations in plasma concentrations of cholecystokinin and gastrin in man

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  2. Validation of suPAR turbidimetric assay on Cobas® (c502 and c702) and comparison to suPAR ELISA

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  3. Transcerebral exchange kinetics of large neutral amino acids during acute inspiratory hypoxia in humans

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  4. Platelet function in lung cancer patients undergoing lobectomy

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  1. Oral D/L-3-Hydroxybutyrate stimulates cholecystokinin and insulin secretion and slows gastric emptying in healthy males

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  2. Bilio-enteric flow and plasma concentrations of bile acids after gastric bypass and sleeve gastrectomy

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  3. Circadian variations in plasma concentrations of cholecystokinin and gastrin in man

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  4. Increased oral sodium chloride intake in humans amplifies selectively postprandial GLP-1 but not GIP, CCK, and gastrin in plasma

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Peptide hormones may occur in particularly low amounts in samples from small animals. Hence, in a rat microdialysis study conventional immunoassays were not sufficiently sensitive to measure gastrin in the dialysis samples. We therefore exploited the observation that antibodies raised against the homologous hormone cholecystokinin (CCK) occasionally bind gastrin peptides with significantly higher affinity than the proper ligand. The immunoassay thus established could detect 1.0 pmol/l in 15 μl microdialysate, which corresponds to 23 attomol gastrin. Such detection limit is five-fold lower than that obtained with the most avid conventional gastrin antibodies. The results may encourage similar approaches for other peptides using homologue-raised antibodies when supersensitivity is required.
Original languageEnglish
JournalScandinavian Journal of Clinical & Laboratory Investigation
Volume72
Issue number2
Pages (from-to)175-9
Number of pages5
ISSN0036-5513
DOIs
Publication statusPublished - 2012

    Research areas

  • Amino Acid Sequence, Animals, Antibodies, Cholecystokinin, Gastrins, Limit of Detection, Microdialysis, Molecular Sequence Data, Rabbits, Radioimmunoassay

ID: 36789887