Harvard
Lennartz, F, Adams, Y
, Bengtsson, A, Olsen, RW, Turner, L, Ndam, NT, Ecklu-Mensah, G, Moussiliou, A, Ofori, MF, Gamain, B
, Lusingu, JP, Petersen, JEV, Wang, CW, Nunes-Silva, S
, Jespersen, JS, Lau, CKY
, Theander, TG, Lavstsen, T, Hviid, L, Higgins, MK
& Jensen, ATR 2017, '
Structure-Guided Identification of a Family of Dual Receptor-Binding PfEMP1 that Is Associated with Cerebral Malaria'
Cell Host and Microbe, vol. 21, no. 3, pp. 403-414.
https://doi.org/10.1016/j.chom.2017.02.009APA
Lennartz, F., Adams, Y.
, Bengtsson, A., Olsen, R. W., Turner, L., Ndam, N. T.
, ... Jensen, A. T. R. (2017).
Structure-Guided Identification of a Family of Dual Receptor-Binding PfEMP1 that Is Associated with Cerebral Malaria.
Cell Host and Microbe,
21(3), 403-414.
https://doi.org/10.1016/j.chom.2017.02.009CBE
Lennartz F, Adams Y
, Bengtsson A, Olsen RW, Turner L, Ndam NT, Ecklu-Mensah G, Moussiliou A, Ofori MF, Gamain B
, Lusingu JP, Petersen JEV, Wang CW, Nunes-Silva S
, Jespersen JS, Lau CKY
, Theander TG, Lavstsen T, Hviid L, Higgins MK
, Jensen ATR. 2017.
Structure-Guided Identification of a Family of Dual Receptor-Binding PfEMP1 that Is Associated with Cerebral Malaria.
Cell Host and Microbe. 21(3):403-414.
https://doi.org/10.1016/j.chom.2017.02.009MLA
Vancouver
Author
Lennartz, Frank ; Adams, Yvonne
; Bengtsson, Anja ; Olsen, Rebecca W ; Turner, Louise ; Ndam, Nicaise T ; Ecklu-Mensah, Gertrude ; Moussiliou, Azizath ; Ofori, Michael F ; Gamain, Benoit
; Lusingu, John P ; Petersen, Jens E V ; Wang, Christian W ; Nunes-Silva, Sofia
; Jespersen, Jakob S ; Lau, Clinton K Y
; Theander, Thor G ; Lavstsen, Thomas ; Hviid, Lars ; Higgins, Matthew K
; Jensen, Anja T R. /
Structure-Guided Identification of a Family of Dual Receptor-Binding PfEMP1 that Is Associated with Cerebral Malaria. In:
Cell Host and Microbe. 2017 ; Vol. 21, No. 3. pp. 403-414.
Bibtex
@article{f72d910a49bf49dba4e9ca07bf4e44f1,
title = "Structure-Guided Identification of a Family of Dual Receptor-Binding PfEMP1 that Is Associated with Cerebral Malaria",
abstract = "Cerebral malaria is a deadly outcome of infection by Plasmodium falciparum, occurring when parasite-infected erythrocytes accumulate in the brain. These erythrocytes display parasite proteins of the PfEMP1 family that bind various endothelial receptors. Despite the importance of cerebral malaria, a binding phenotype linked to its symptoms has not been identified. Here, we used structural biology to determine how a group of PfEMP1 proteins interacts with intercellular adhesion molecule 1 (ICAM-1), allowing us to predict binders from a specific sequence motif alone. Analysis of multiple Plasmodium falciparum genomes showed that ICAM-1-binding PfEMP1s also interact with endothelial protein C receptor (EPCR), allowing infected erythrocytes to synergistically bind both receptors. Expression of these PfEMP1s, predicted to bind both ICAM-1 and EPCR, is associated with increased risk of developing cerebral malaria. This study therefore reveals an important PfEMP1-binding phenotype that could be targeted as part of a strategy to prevent cerebral malaria.",
keywords = "Antigens, CD, Cell Adhesion, Computational Biology, Crystallography, X-Ray, Endothelial Protein C Receptor, Genome, Protozoan, Intercellular Adhesion Molecule-1, Malaria, Cerebral, Malaria, Falciparum, Plasmodium falciparum, Protein Binding, Protozoan Proteins, Receptors, Cell Surface, Scattering, Small Angle, Sequence Analysis, DNA, Surface Plasmon Resonance, Virulence Factors, Journal Article",
author = "Frank Lennartz and Yvonne Adams and Anja Bengtsson and Olsen, {Rebecca W} and Louise Turner and Ndam, {Nicaise T} and Gertrude Ecklu-Mensah and Azizath Moussiliou and Ofori, {Michael F} and Benoit Gamain and Lusingu, {John P} and Petersen, {Jens E V} and Wang, {Christian W} and Sofia Nunes-Silva and Jespersen, {Jakob S} and Lau, {Clinton K Y} and Theander, {Thor G} and Thomas Lavstsen and Lars Hviid and Higgins, {Matthew K} and Jensen, {Anja T R}",
note = "Copyright {\circledC} 2017 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2017",
month = "3",
day = "8",
doi = "10.1016/j.chom.2017.02.009",
language = "English",
volume = "21",
pages = "403--414",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "3",
}
RIS
TY - JOUR
T1 - Structure-Guided Identification of a Family of Dual Receptor-Binding PfEMP1 that Is Associated with Cerebral Malaria
AU - Lennartz, Frank
AU - Adams, Yvonne
AU - Bengtsson, Anja
AU - Olsen, Rebecca W
AU - Turner, Louise
AU - Ndam, Nicaise T
AU - Ecklu-Mensah, Gertrude
AU - Moussiliou, Azizath
AU - Ofori, Michael F
AU - Gamain, Benoit
AU - Lusingu, John P
AU - Petersen, Jens E V
AU - Wang, Christian W
AU - Nunes-Silva, Sofia
AU - Jespersen, Jakob S
AU - Lau, Clinton K Y
AU - Theander, Thor G
AU - Lavstsen, Thomas
AU - Hviid, Lars
AU - Higgins, Matthew K
AU - Jensen, Anja T R
N1 - Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved.
PY - 2017/3/8
Y1 - 2017/3/8
N2 - Cerebral malaria is a deadly outcome of infection by Plasmodium falciparum, occurring when parasite-infected erythrocytes accumulate in the brain. These erythrocytes display parasite proteins of the PfEMP1 family that bind various endothelial receptors. Despite the importance of cerebral malaria, a binding phenotype linked to its symptoms has not been identified. Here, we used structural biology to determine how a group of PfEMP1 proteins interacts with intercellular adhesion molecule 1 (ICAM-1), allowing us to predict binders from a specific sequence motif alone. Analysis of multiple Plasmodium falciparum genomes showed that ICAM-1-binding PfEMP1s also interact with endothelial protein C receptor (EPCR), allowing infected erythrocytes to synergistically bind both receptors. Expression of these PfEMP1s, predicted to bind both ICAM-1 and EPCR, is associated with increased risk of developing cerebral malaria. This study therefore reveals an important PfEMP1-binding phenotype that could be targeted as part of a strategy to prevent cerebral malaria.
AB - Cerebral malaria is a deadly outcome of infection by Plasmodium falciparum, occurring when parasite-infected erythrocytes accumulate in the brain. These erythrocytes display parasite proteins of the PfEMP1 family that bind various endothelial receptors. Despite the importance of cerebral malaria, a binding phenotype linked to its symptoms has not been identified. Here, we used structural biology to determine how a group of PfEMP1 proteins interacts with intercellular adhesion molecule 1 (ICAM-1), allowing us to predict binders from a specific sequence motif alone. Analysis of multiple Plasmodium falciparum genomes showed that ICAM-1-binding PfEMP1s also interact with endothelial protein C receptor (EPCR), allowing infected erythrocytes to synergistically bind both receptors. Expression of these PfEMP1s, predicted to bind both ICAM-1 and EPCR, is associated with increased risk of developing cerebral malaria. This study therefore reveals an important PfEMP1-binding phenotype that could be targeted as part of a strategy to prevent cerebral malaria.
KW - Antigens, CD
KW - Cell Adhesion
KW - Computational Biology
KW - Crystallography, X-Ray
KW - Endothelial Protein C Receptor
KW - Genome, Protozoan
KW - Intercellular Adhesion Molecule-1
KW - Malaria, Cerebral
KW - Malaria, Falciparum
KW - Plasmodium falciparum
KW - Protein Binding
KW - Protozoan Proteins
KW - Receptors, Cell Surface
KW - Scattering, Small Angle
KW - Sequence Analysis, DNA
KW - Surface Plasmon Resonance
KW - Virulence Factors
KW - Journal Article
U2 - 10.1016/j.chom.2017.02.009
DO - 10.1016/j.chom.2017.02.009
M3 - Journal article
VL - 21
SP - 403
EP - 414
JO - Cell Host and Microbe
JF - Cell Host and Microbe
SN - 1931-3128
IS - 3
ER -
