TY - JOUR
T1 - Stop-and-go kinetics in amyloid fibrillation
AU - Ferkinghoff-Borg, Jesper
AU - Fonslet, J.
AU - Andersen, C.B.
AU - Krishna, S.
AU - Pigolotti, Simone
AU - Yagi, H.
AU - Goto, Y.
AU - Otzen, Daniel
AU - Jensen, M.H.
PY - 2010
Y1 - 2010
N2 - Many human diseases are associated with protein aggregation and fibrillation. We present experiments on in vitro glucagon fibrillation using total internal reflection fluorescence microscopy, providing real-time measurements of single-fibril growth. We find that amyloid fibrils grow in an intermittent fashion, with periods of growth followed by long pauses. The observed exponential distributions of stop and growth times support a Markovian model, in which fibrils shift between the two states with specific rates. Even if the individual rates vary considerably, we observe that the probability of being in the growing (stopping) state is very close to 1/4 (3/4) in all experiments
AB - Many human diseases are associated with protein aggregation and fibrillation. We present experiments on in vitro glucagon fibrillation using total internal reflection fluorescence microscopy, providing real-time measurements of single-fibril growth. We find that amyloid fibrils grow in an intermittent fashion, with periods of growth followed by long pauses. The observed exponential distributions of stop and growth times support a Markovian model, in which fibrils shift between the two states with specific rates. Even if the individual rates vary considerably, we observe that the probability of being in the growing (stopping) state is very close to 1/4 (3/4) in all experiments
U2 - 10.1103/PhysRevE.82.010901
DO - 10.1103/PhysRevE.82.010901
M3 - Journal article
C2 - 20866557
SN - 1539-3755
VL - 82
JO - Physical Review E (Statistical, Nonlinear, and Soft Matter Physics)
JF - Physical Review E (Statistical, Nonlinear, and Soft Matter Physics)
IS - 1
ER -