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Smoking reduces circulating CD26hiCD161hi MAIT cells in healthy individuals and patients with multiple sclerosis

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DOI

  1. Niels Borregaard, M.D. (1951-2017)

    Research output: Contribution to journalJournal articleCommunication

  2. CD4+/CD8+ double-positive T cells: more than just a developmental stage?

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Molecular and stimulus-response profiles illustrate heterogeneity between peripheral and cord blood-derived human mast cells

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  4. Candida albicans escapes from mouse neutrophils

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Diagnostic Value of Oligoclonal Bands in Children: A Nationwide Population-Based Cohort Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Smoking is associated with increased disease activity during natalizumab treatment in multiple sclerosis

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Genome-wide gene expression in a pharmacological hormonal transition model and its relation to depressive symptoms

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Functional neuroimaging of recovery from motor conversion disorder: A case report

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Upon chronic cigarette smoke exposure, inhaled antigens and irritants cause altered lung immune homeostasis. Circulating immune cells are affected, and smoking is associated with an increased risk of developing various disorders, including multiple sclerosis (MS). This study was conducted to determine the impact of smoking on circulating immune cell subsets. Furthermore, we determined whether any smoking-associated changes were related to MS. With the use of flow cytometry, CFSE assays, and ELISpot assays, we analyzed circulating immune cell phenotypes and quantified antigen-induced proliferation and cytokine secretion in smokers and nonsmokers in a cohort of 100 healthy individuals (HI). In addition, we analyzed immune cell subsets associated with smoking in 2 independent cohorts of patients with MS. In HI smokers compared with nonsmokers, we found increased blood cell counts of granulocytes, monocytes, and lymphocytes. These cells were not more proinflammatory, autoreactive, or EBV reactive compared with cells from nonsmokers. Phenotypic differences were seen in plasmacytoid dendritic cells (pDCs) and CD8+ T cells as higher percentages of ICOS ligand (ICOSL)+ pDCs and lower percentages of CD26hiCD161hi CD8+ T cells and CCR6+ CD8+ T cells in smokers compared with nonsmokers. In supplemental analyses, we showed that CD26hiCD161hi CD8+ T cells were mainly mucosal-associated invariant T cells (MAITs). Comparable frequencies of ICOSL+ pDCs, CCR6+ CD8+ T cells, and CD26hiCD161hi CD8+ T cells were found between HI and MS patients who were nonsmokers. Our findings suggest general proinflammatory effects from smoking combined with skewing of specific cell populations in HI and MS patients. The function of these cell populations needs further investigation.

Original languageEnglish
JournalJournal of Leukocyte Biology
Volume101
Issue number5
Pages (from-to)1211-1220
Number of pages10
ISSN0741-5400
DOIs
Publication statusPublished - May 2017

    Research areas

  • Adult, CD8-Positive T-Lymphocytes, Cell Count, Cohort Studies, Cotinine, Dendritic Cells, Dipeptidyl Peptidase 4, Female, Gene Expression Regulation, Granulocytes, Humans, Immunophenotyping, Inducible T-Cell Co-Stimulator Ligand, Male, Middle Aged, Monocytes, Multiple Sclerosis, NK Cell Lectin-Like Receptor Subfamily B, Primary Cell Culture, Smoking, Journal Article, Research Support, Non-U.S. Gov't

ID: 52217022