Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Sex-specific estrogen levels and reference intervals from infancy to late adulthood determined by LC-MS/MS

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Dynamic changes of reproductive hormones in male minipuberty: Temporal dissociation of Leydig- and Sertoli-cell activity

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Severe mental illness and the risk of diabetes complications. A nationwide register-based cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Patients with cirrhosis have elevated bone turnover but normal hepatic production of osteoprotegerin

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Lipoprotein(a) levels at birth and in early childhood: The COMPARE Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Colonic lactulose fermentation has no impact on glucagon-like peptide-1 and peptide-YY secretion in healthy young men

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Dynamic changes of reproductive hormones in male minipuberty: Temporal dissociation of Leydig- and Sertoli-cell activity

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Oophorectomy and rate of dementia: a prospective cohort study

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Biochemical abnormalities among patients referred for celiac disease antibody blood testing in a primary health care setting

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Pregnancy exposure to bisphenol A and duration of breastfeeding

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Risk Factors, Subsequent Disease Onset, and Prognostic Impact of Myocardial Infarction and Atrial Fibrillation

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

CONTEXT: The lack of sensitive and robust analytical methods has hindered the reliable quantification of estrogen metabolites in subjects with low concentrations.

OBJECTIVE: To establish sex-specific reference ranges for estrone (E1) and estradiol (E2) throughout life and to evaluate sex-differences using the state-of-the-art liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of E1, E2, and estriol (E3).

DESIGN: LC-MS/MS method development and construction of estrogen reference ranges.

SETTINGS: Population-based cross-sectional cohorts from the greater Copenhagen and Aarhus areas.

PARTICIPANTS: Healthy participants aged 3 months to 61 years (n = 1838).

RESULTS: An isotope diluted LC-MS/MS method was developed and validated for measurements of serum E1, E2, and E3. Limits of detections (LODs) were 3 pmol/L (E1), 4 pmol/L (E2), and 12 pmol/L (E3), respectively. This sensitive method made it possible to differentiate between male and female concentration levels of E1 and E2 in children. In girls, E2 levels ranged from <LOD to 100 pmol/L during mini-puberty, whereas it was ≤20 pmol/L during childhood. E1 and E2 increased with age and pubertal breast stage and varied during the menstrual cycle; E1 was lower than E2 in girls and premenopausal women, and higher than E2 in postmenopausal women. In boys, E1 and E2 increased with age and pubertal stage, whereas little changes with age were observed in men. High E3 concentrations were confirmed in pregnant women.

CONCLUSION: Reference ranges of simultaneous quantification of E1 and E2 by this novel specific and highly sensitive LC-MS/MS method provide an invaluable tool in clinical practice and in future research studies.

Original languageEnglish
JournalThe Journal of clinical endocrinology and metabolism
Volume105
Issue number3
Pages (from-to)754-768
ISSN0021-972X
DOIs
Publication statusPublished - 1 Mar 2020

    Research areas

  • childhood, estradiol, estrone, LC-MS/MS, menopause, menstrual cycle, mini-puberty, pubertal development, reference range

ID: 58366501