Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Serum levels of INSL3, AMH, Inhibin B and Testosterone during pubertal transition in healthy boys

Research output: Contribution to journalJournal articleResearch

DOI

  1. Freezing of ovarian tissue and clinical opportunities

    Research output: Contribution to journalReviewResearchpeer-review

  2. Role of Nodal signalling in testis development and initiation of testicular cancer

    Research output: Contribution to journalReviewResearchpeer-review

  3. Prenatal exposure to paracetamol/acetaminophen and precursor aniline impairs masculinisation of male brain and behaviour

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Phosphodiesterases in the rat ovary - effect of cAMP in primordial follicles

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. In utero exposure to persistent organochlorine pollutants and reproductive health in the human male

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. Evaluation of Serum Insulin-like Factor 3 Quantification by LC-MS/MS as a Biomarker of Leydig Cell Function

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Luteinizing Hormone Receptor Is Expressed in Testicular Germ Cell Tumors: Possible Implications for Tumor Growth and Prognosis

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Use of stored serum in the study of time trends and geographical differences in exposure of pregnant women to phthalates

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations
IntroductionInsulin-like factor 3 (INSL3) is a promising marker of Leydig cell function with potentially high clinical relevance. Limited data of INSL3 levels in relation to other reproductive hormones in healthy pubertal boys exist.AimTo evaluate longitudinal serum changes in INSL3 compared to luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone, inhibin B, and anti-Müllerian hormone (AMH) during puberty in healthy boys.MethodsTen boys were included from the longitudinal part of the COPENHAGEN Puberty Study. Pubertal evaluation, including testicular volume, was performed and blood samples drawn every 6 months for 5 years. Serum concentrations of testosterone were determined by a newly developed LC-MS/MS method, and serum concentrations of INSL3, AMH, inhibin B, FSH and LH, respectively, were determined by validated immunoassays.ResultsSerum INSL3 levels increased progressively with increasing age, pubertal onset and testicular volume. In six of ten boys, LH increased prior to the first observed increase in INSL3. In the remaining four boys, the increase in LH and INSL3 was observed at the same examination. The increases in serum concentrations of LH, testosterone and INSL3 were not parallel or in ordered succession and varied inter-individuallyConclusionWe demonstrated that INSL3 concentrations were tightly associated with pubertal onset and increasing testicular volume. However, the pubertal increases in LH, INSL3, and testosterone concentrations were not entirely parallel, suggesting that INSL3 and testosterone may be regulated differently. Thus, we speculate that INSL3 provides additional information on Leydig cell differentiation and function during puberty compared with traditional markers of testicular function.
Original languageEnglish
JournalReproduction (Cambridge, England)
Volume147
Pages (from-to)529-535
ISSN1470-1626
DOIs
Publication statusPublished - 23 Jan 2014

ID: 42608324