Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Serum insulin-like factor 3 levels are reduced in former androgen users suggesting impaired Leydig cell capacity

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Expression of cholecystokinin and its receptors in the intestinal tract of type 2 diabetes patients and healthy controls

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Elevated apolipoprotein A1 and HDL cholesterol associated with age-related macular degeneration: 2 population cohorts

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Prenatal Exposure to Butyl Paraben Is Associated With Fat Percentage in 7-Year-Old Boys

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Treatment of Hyperthyroidism Reduces Systemic Oxidative Stress, as Measured by Markers of RNA and DNA Damage

    Research output: Contribution to journalJournal articleResearchpeer-review

  5. Incidence and Clinical Presentation of Pheochromocytoma and Sympathetic Paraganglioma: A Population-based Study

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. The effects of selected inhibitors on human fetal adrenal steroidogenesis differs under basal and ACTH-stimulated conditions

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Cohort profile: The COPENHAGEN Minipuberty Study-A longitudinal prospective cohort of healthy full-term infants and their parents

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Variant PNLDC1, Defective piRNA Processing, and Azoospermia

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Effects of Empagliflozin on Myocardial Flow Reserve in Patients With Type 2 Diabetes Mellitus: The SIMPLE Trial

    Research output: Contribution to journalJournal articleResearchpeer-review

View graph of relations

CONTEXT: Illicit use of anabolic androgenic steroids (AAS) is frequently observed in men and is associated with subsequent testosterone deficiency although the long-term effect on gonadal function is still unclear. Serum insulin-like factor 3 (INSL3) has been suggested to be a superior biomarker of Leydig cell secretory capacity compared to testosterone.

OBJECTIVE: This study aimed to investigate serum INSL3 concentrations in AAS users.

METHODS: This community-based, cross-sectional study included men aged 18 to 50 years, involved in recreational strength training and allocated to 1 of 3 groups: never-AAS users as controls (n = 44), current (n = 46), or former AAS users (n = 42) with an average duration since AAS cessation of 32 (23 ; 45) months.

RESULTS: Serum INSL3 was lower in current AAS users and former AAS users than in controls, median (interquartile range), 0.04 µg/L (nondetectable [ND]-0.07 µg/L) and 0.39 µg/L (0.24-0.62 µg/L) vs 0.59 µg/L (0.45-0.72 µg/L), P less than .001. Former AAS users exhibited lower serum INSL3 levels than controls in a multivariable linear regression even after adjusting for serum total testosterone (TT) and other relevant confounders, (B) (95% CI), -0.16 µg/L (95% CI, -0.29 to -0.04 µg/L), P equal to .011. INSL3 and TT were not associated in the model, P equal to .821. Longer accumulated AAS duration (log2) was associated with lower serum INSL3 in former AAS users, (B) (95% CI), -0.08 (95% CI, -0.14 to -0.01), P equal to .022. Serum INSL3, but not inhibin B or testosterone, was associated with testicular size in a multivariate linear regression, (B) (95% CI); 4.7 (95% CI, 0.5 to 8.9), P equal to .030.

CONCLUSION: Serum INSL3 is reduced years following AAS cessation in men, independently of testosterone, suggesting persistently impaired Leydig cell capacity.

Original languageEnglish
JournalThe Journal of clinical endocrinology and metabolism
Volume106
Issue number7
Pages (from-to)e2664-e2672
ISSN0021-972X
DOIs
Publication statusPublished - 16 Jun 2021

    Research areas

  • anabolic androgenic steroids, androgens, insulin-like factor 3, male hypogonadism, male infertility, testosterone

ID: 64128407