Research
Print page Print page
Switch language
The Capital Region of Denmark - a part of Copenhagen University Hospital
Published

Seromic profiling of colorectal cancer patients with novel glycopeptide microarray

Research output: Contribution to journalJournal articleResearchpeer-review

DOI

  1. Testicular cancer incidence predictions in Europe 2010–2035: A rising burden despite population ageing

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Menstrual pain and risk of epithelial ovarian cancer: Results from the Ovarian Cancer Association Consortium

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Tibolone and risk of gynecological hormone sensitive cancer

    Research output: Contribution to journalJournal articleResearchpeer-review

  4. Does HPV status influence survival after vulvar cancer?

    Research output: Contribution to journalJournal articleResearchpeer-review

  1. CDX2 downregulation is associated with poor differentiation and MMR deficiency in colon cancer

    Research output: Contribution to journalJournal articleResearchpeer-review

  2. Laparoscopic lavage is feasible and safe for the treatment of perforated diverticulitis with purulent peritonitis

    Research output: Contribution to journalJournal articleResearchpeer-review

  3. Watchful waiting versus colorectal resection after polypectomy for malignant colorectal polyps

    Research output: Contribution to journalJournal articleResearchpeer-review

  • Johannes Wirenfeldt Vad Pedersen
  • Ola Blixt
  • Eric Paul Bennett
  • Mads Peter Agervig Tarp
  • Imran Parvez Dar
  • Ulla Mandel
  • Steen Seier Poulsen
  • Anders E Pedersen
  • Susanne Rasmussen
  • Per Jess
  • Henrik Clausen
  • Hans H. Wandall
View graph of relations
Cancer-associated autoantibodies hold promise as sensitive biomarkers for early detection of cancer. Aberrant post-translational variants of proteins are likely to induce autoantibodies, and changes in O-linked glycosylation represent one of the most important cancer-associated post-translational modifications (PTMs). Short aberrant O-glycans on proteins may introduce novel glycopeptide epitopes that can elicit autoantibodies because of lack of tolerance. Technical barriers, however, have hampered detection of such glycopeptide-specific autoantibodies. Here, we have constructed an expanded glycopeptide array displaying a comprehensive library of glycopeptides and glycoproteins derived from a panel of human mucins (MUC1, MUC2, MUC4, MUC5AC, MUC6 and MUC7) known to have altered glycosylation and expression in cancer. Seromic profiling of patients with colorectal cancer identified cancer-associated autoantibodies to a set of aberrant glycopeptides derived from MUC1 and MUC4. The cumulative sensitivity of the array analysis was 79% with a specificity of 92%. The most prevalent of the identified autoantibody targets were validated as authentic cancer immunogens by showing expression of the epitopes in cancer using novel monoclonal antibodies. Our study provides evidence for the value of glycopeptides and other PTM-peptide arrays in diagnostic measures.
Original languageEnglish
JournalInternational Journal of Cancer
Volume128
Issue number8
Pages (from-to)1860-71
Number of pages12
ISSN0020-7136
DOIs
Publication statusPublished - 15 Apr 2011

ID: 31038640