Sequenced response of extracellular matrix deadhesion and fibrotic regulators after muscle damage is involved in protection against future injury in human skeletal muscle

Abigail L Mackey, Simon Brandstetter, Peter Schjerling, Jens Bojsen-Moller, Klaus Qvortrup, Mette M Pedersen, Simon Doessing, Michael Kjaer, S Peter Magnusson, Henning Langberg

144 Citations (Scopus)

Abstract

The purpose of this study was to test the hypothesis that remodeling of skeletal muscle extracellular matrix (ECM) is involved in protecting human muscle against injury. Biopsies were obtained from medial gastrocnemius muscles after a single bout of electrical stimulation (B) or a repeated bout (RB) 30 d later, or 30 d after a single stimulation bout (RBc). A muscle biopsy was collected from the control leg for comparison with the stimulated leg. Satellite cell content, tenascin C, and muscle regeneration were assessed by immunohistochemistry; real-time PCR was used to measure mRNA levels of collagens, laminins, heat-shock proteins (HSPs), inflammation, and related growth factors. The large responses of HSPs, CCL2, and tenascin C detected 48 h after a single bout were attenuated in the RB trial, indicative of protection against injury. Satellite cell content and 12 target genes, including IGF-1, were elevated 30 d after a single bout. Among those displaying the greatest difference vs. control muscle, ECM laminin-β1 and collagen types I and III were elevated ∼6- to 9-fold (P
Original languageEnglish
JournalF A S E B Journal
Volume25
Issue number6
Pages (from-to)1943-59
Number of pages17
ISSN0892-6638
DOIs
Publication statusPublished - 2011

Keywords

  • Adult
  • Collagen Type I
  • Collagen Type II
  • Electric Stimulation
  • Extracellular Matrix
  • Fibrosis
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Humans
  • Macrophages
  • Male
  • Muscle, Skeletal
  • Muscular Diseases
  • Polymerase Chain Reaction
  • Regeneration
  • Tenascin
  • Young Adult

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