Separation of steroid isomers by ion mobility mass spectrometry

Linda Ahonen; Maíra Fasciotti; Gustav Boije af Gennäs; Tapio Kotiaho; Romeu J. Daroda; Marcos Eberlin; Risto Kostiainen

doi:10.1016/j.chroma.2013.08.056

Separation of steroid isomers by ion mobility mass spectrometry

Linda Ahonen^*, Maíra Fasciotti, Gustav Boije af Gennäs, Tapio Kotiaho, Romeu J. Daroda, Marcos Eberlin, Risto Kostiainen

^*Corresponding author for this work

83 Citations (Scopus)

Abstract

Ion mobility mass spectrometry performed in a compact traveling wave cell (TWIM-MS) is shown to provide a reliable, fast and repeatable method to separate derivatized steroid isomers. Three steroid isomer pairs were analyzed in their native form and as their p-toluenesulfonyl isocyanate derivatives. The native steroids were separated from each other, but no separation could be attained for the isomers. The derivatized steroid isomers were, however, properly separated by TWIM-MS with peak-to-peak resolutions close to or as high as baseline resolution (R_p-p=0.77-1.08).

Original language	English
Journal	Journal of Chromatography A
Volume	1310
Pages (from-to)	133-137
Number of pages	5
ISSN	0021-9673
DOIs	https://doi.org/10.1016/j.chroma.2013.08.056
Publication status	Published - 4 Oct 2013
Externally published	Yes

Keywords

Collision cross section
Derivatization
Ion mobility mass spectrometry
Steroid isomers
Steroids

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10.1016/j.chroma.2013.08.056

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title = "Separation of steroid isomers by ion mobility mass spectrometry",

abstract = "Ion mobility mass spectrometry performed in a compact traveling wave cell (TWIM-MS) is shown to provide a reliable, fast and repeatable method to separate derivatized steroid isomers. Three steroid isomer pairs were analyzed in their native form and as their p-toluenesulfonyl isocyanate derivatives. The native steroids were separated from each other, but no separation could be attained for the isomers. The derivatized steroid isomers were, however, properly separated by TWIM-MS with peak-to-peak resolutions close to or as high as baseline resolution (Rp-p=0.77-1.08).",

keywords = "Collision cross section, Derivatization, Ion mobility mass spectrometry, Steroid isomers, Steroids",

author = "Linda Ahonen and Ma{\'i}ra Fasciotti and Genn{\"a}s, {Gustav Boije af} and Tapio Kotiaho and Daroda, {Romeu J.} and Marcos Eberlin and Risto Kostiainen",

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doi = "10.1016/j.chroma.2013.08.056",

language = "English",

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T1 - Separation of steroid isomers by ion mobility mass spectrometry

AU - Ahonen, Linda

AU - Fasciotti, Maíra

AU - Gennäs, Gustav Boije af

AU - Kotiaho, Tapio

AU - Daroda, Romeu J.

AU - Eberlin, Marcos

AU - Kostiainen, Risto

PY - 2013/10/4

Y1 - 2013/10/4

N2 - Ion mobility mass spectrometry performed in a compact traveling wave cell (TWIM-MS) is shown to provide a reliable, fast and repeatable method to separate derivatized steroid isomers. Three steroid isomer pairs were analyzed in their native form and as their p-toluenesulfonyl isocyanate derivatives. The native steroids were separated from each other, but no separation could be attained for the isomers. The derivatized steroid isomers were, however, properly separated by TWIM-MS with peak-to-peak resolutions close to or as high as baseline resolution (Rp-p=0.77-1.08).

AB - Ion mobility mass spectrometry performed in a compact traveling wave cell (TWIM-MS) is shown to provide a reliable, fast and repeatable method to separate derivatized steroid isomers. Three steroid isomer pairs were analyzed in their native form and as their p-toluenesulfonyl isocyanate derivatives. The native steroids were separated from each other, but no separation could be attained for the isomers. The derivatized steroid isomers were, however, properly separated by TWIM-MS with peak-to-peak resolutions close to or as high as baseline resolution (Rp-p=0.77-1.08).

KW - Collision cross section

KW - Derivatization

KW - Ion mobility mass spectrometry

KW - Steroid isomers

KW - Steroids

UR - http://www.scopus.com/inward/record.url?scp=84883789257&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2013.08.056

DO - 10.1016/j.chroma.2013.08.056

M3 - Journal article

AN - SCOPUS:84883789257

SN - 0021-9673

VL - 1310

SP - 133

EP - 137

JO - Journal of Chromatography A

JF - Journal of Chromatography A

ER -

Separation of steroid isomers by ion mobility mass spectrometry

Abstract

Keywords

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