Selection of high quality spermatozoa may be promoted by activated vitamin D in the woman

Lasse Bøllehuus Hansen; Anders Rehfeld; Rosanna de Neergaard; John Erik Nielsen; Lea Hedegaard Iversen; Ida Marie Boisen; Li Juel Mortensen; Beate Lanske; Kristian Almstrup; Elisabeth Carlsen; Anders Hayden Berg; Niels Jørgensen; Anders Nyboe Andersen; Anders Juul; Martin Blomberg Jensen

doi:10.1210/jc.2016-3008

Selection of high quality spermatozoa may be promoted by activated vitamin D in the woman

Lasse Bøllehuus Hansen, Anders Rehfeld, Rosanna de Neergaard, John Erik Nielsen, Lea Hedegaard Iversen, Ida Marie Boisen, Li Juel Mortensen, Beate Lanske, Kristian Almstrup, Elisabeth Carlsen, Anders Hayden Berg, Niels Jørgensen, Anders Nyboe Andersen, Anders Juul, Martin Blomberg Jensen

25 Citations (Scopus)

Abstract

CONTEXT: The vitamin D receptor (VDR) and enzymes involved in activation (CYP2R1, CYP27B1) and inactivation (CYP24A1) of vitamin D are expressed in ovary, testes, and spermatozoa.

OBJECTIVE: Determine responsiveness to 1,25-dihydroxyvitamin D (1,25(OH)2D3) in spermatozoa from normal and infertile men, identify site of exposure and how 1,25(OH)2D3 influences sperm function.

DESIGN: Spermatozoa expressing VDR, CYP2R1, CYP27B1, and CYP24A1 were analyzed in normal and infertile men. 25-hydroxyvitamin D (25-OHD), 24,25-dihydroxyvitamin D (24,25(OH)2D3), and 1,25(OH)2D3 were measured in serum, seminal fluid, cervical secretions and ovarian follicular fluid. 1,25(OH)2D3 was tested on human spermatozoa.

SETTING: Tertiary centre for fertility.

PARTICIPANTS: Protein expression in spermatozoa and semen quality were assessed in 230 infertile and 114 healthy men. Vitamin D metabolites were measured in fluids from 245 men and 13 women, while 74 oocytes and 17 semen donors were used for sperm-function tests.

MAIN OUTCOME MEASURES: VDR and CYP24A1 expressions in spermatozoa, fluid concentrations of 25-OHD, 24,25(OH)2D3 and 1,25(OH)2D3, and 1,25(OH)2D3-induced effects on intracellular calcium concentration ([Ca(2+)]i) and sperm-oocyte binding in vitro.

RESULTS: VDR and CYP24A1 were expressed in >2 fold higher fraction of spermatozoa from normal than infertile men (p<0.01). Concentrations of 25-OHD, 24,25(OH)2D, and 1,25(OH)2D3 were undetectable in seminal fluid but high in ovarian follicular fluid. Follicular concentrations of 1,25(OH)2D3 induced a modest increase in [Ca(2+)]i and sperm-oocyte binding in vitro (p<0.05).

CONCLUSION: Presence of VDR and CYP24A1 mainly in spermatozoa of higher quality supports that 1,25(OH)2D3 available in the female reproductive tract may promote selection of the best gametes for fertilization.

Original language	English
Journal	The Journal of clinical endocrinology and metabolism
Volume	102
Issue number	3
Pages (from-to)	950-61
ISSN	0021-972X
DOIs	https://doi.org/10.1210/jc.2016-3008
Publication status	Published - 2017

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10.1210/jc.2016-3008

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Bøllehuus Hansen, L., Rehfeld, A., de Neergaard, R., Nielsen, J. E., Iversen, L. H., Boisen, I. M., Mortensen, L. J., Lanske, B., Almstrup, K., Carlsen, E., Berg, A. H., Jørgensen, N., Andersen, A. N., Juul, A., & Blomberg Jensen, M. (2017). Selection of high quality spermatozoa may be promoted by activated vitamin D in the woman. The Journal of clinical endocrinology and metabolism, 102(3), 950-61. https://doi.org/10.1210/jc.2016-3008

Bøllehuus Hansen, L, Rehfeld, A, de Neergaard, R, Nielsen, JE, Iversen, LH, Boisen, IM, Mortensen, LJ, Lanske, B, Almstrup, K , Carlsen, E, Berg, AH, Jørgensen, N, Andersen, AN, Juul, A & Blomberg Jensen, M 2017, 'Selection of high quality spermatozoa may be promoted by activated vitamin D in the woman', The Journal of clinical endocrinology and metabolism, vol. 102, no. 3, pp. 950-61. https://doi.org/10.1210/jc.2016-3008

@article{fea1174603c94626ae6008b2d22646a6,

title = "Selection of high quality spermatozoa may be promoted by activated vitamin D in the woman",

abstract = "CONTEXT: The vitamin D receptor (VDR) and enzymes involved in activation (CYP2R1, CYP27B1) and inactivation (CYP24A1) of vitamin D are expressed in ovary, testes, and spermatozoa.OBJECTIVE: Determine responsiveness to 1,25-dihydroxyvitamin D (1,25(OH)2D3) in spermatozoa from normal and infertile men, identify site of exposure and how 1,25(OH)2D3 influences sperm function.DESIGN: Spermatozoa expressing VDR, CYP2R1, CYP27B1, and CYP24A1 were analyzed in normal and infertile men. 25-hydroxyvitamin D (25-OHD), 24,25-dihydroxyvitamin D (24,25(OH)2D3), and 1,25(OH)2D3 were measured in serum, seminal fluid, cervical secretions and ovarian follicular fluid. 1,25(OH)2D3 was tested on human spermatozoa.SETTING: Tertiary centre for fertility.PARTICIPANTS: Protein expression in spermatozoa and semen quality were assessed in 230 infertile and 114 healthy men. Vitamin D metabolites were measured in fluids from 245 men and 13 women, while 74 oocytes and 17 semen donors were used for sperm-function tests.MAIN OUTCOME MEASURES: VDR and CYP24A1 expressions in spermatozoa, fluid concentrations of 25-OHD, 24,25(OH)2D3 and 1,25(OH)2D3, and 1,25(OH)2D3-induced effects on intracellular calcium concentration ([Ca(2+)]i) and sperm-oocyte binding in vitro.RESULTS: VDR and CYP24A1 were expressed in >2 fold higher fraction of spermatozoa from normal than infertile men (p<0.01). Concentrations of 25-OHD, 24,25(OH)2D, and 1,25(OH)2D3 were undetectable in seminal fluid but high in ovarian follicular fluid. Follicular concentrations of 1,25(OH)2D3 induced a modest increase in [Ca(2+)]i and sperm-oocyte binding in vitro (p<0.05).CONCLUSION: Presence of VDR and CYP24A1 mainly in spermatozoa of higher quality supports that 1,25(OH)2D3 available in the female reproductive tract may promote selection of the best gametes for fertilization.",

author = "\{B{\o}llehuus Hansen\}, Lasse and Anders Rehfeld and \{de Neergaard\}, Rosanna and Nielsen, \{John Erik\} and Iversen, \{Lea Hedegaard\} and Boisen, \{Ida Marie\} and Mortensen, \{Li Juel\} and Beate Lanske and Kristian Almstrup and Elisabeth Carlsen and Berg, \{Anders Hayden\} and Niels J{\o}rgensen and Andersen, \{Anders Nyboe\} and Anders Juul and \{Blomberg Jensen\}, Martin",

year = "2017",

doi = "10.1210/jc.2016-3008",

language = "English",

volume = "102",

pages = "950--61",

journal = "The Journal of clinical endocrinology and metabolism",

issn = "0021-972X",

publisher = "Endocrine Society",

number = "3",

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TY - JOUR

T1 - Selection of high quality spermatozoa may be promoted by activated vitamin D in the woman

AU - Bøllehuus Hansen, Lasse

AU - Rehfeld, Anders

AU - de Neergaard, Rosanna

AU - Nielsen, John Erik

AU - Iversen, Lea Hedegaard

AU - Boisen, Ida Marie

AU - Mortensen, Li Juel

AU - Lanske, Beate

AU - Almstrup, Kristian

AU - Carlsen, Elisabeth

AU - Berg, Anders Hayden

AU - Jørgensen, Niels

AU - Andersen, Anders Nyboe

AU - Juul, Anders

AU - Blomberg Jensen, Martin

PY - 2017

Y1 - 2017

N2 - CONTEXT: The vitamin D receptor (VDR) and enzymes involved in activation (CYP2R1, CYP27B1) and inactivation (CYP24A1) of vitamin D are expressed in ovary, testes, and spermatozoa.OBJECTIVE: Determine responsiveness to 1,25-dihydroxyvitamin D (1,25(OH)2D3) in spermatozoa from normal and infertile men, identify site of exposure and how 1,25(OH)2D3 influences sperm function.DESIGN: Spermatozoa expressing VDR, CYP2R1, CYP27B1, and CYP24A1 were analyzed in normal and infertile men. 25-hydroxyvitamin D (25-OHD), 24,25-dihydroxyvitamin D (24,25(OH)2D3), and 1,25(OH)2D3 were measured in serum, seminal fluid, cervical secretions and ovarian follicular fluid. 1,25(OH)2D3 was tested on human spermatozoa.SETTING: Tertiary centre for fertility.PARTICIPANTS: Protein expression in spermatozoa and semen quality were assessed in 230 infertile and 114 healthy men. Vitamin D metabolites were measured in fluids from 245 men and 13 women, while 74 oocytes and 17 semen donors were used for sperm-function tests.MAIN OUTCOME MEASURES: VDR and CYP24A1 expressions in spermatozoa, fluid concentrations of 25-OHD, 24,25(OH)2D3 and 1,25(OH)2D3, and 1,25(OH)2D3-induced effects on intracellular calcium concentration ([Ca(2+)]i) and sperm-oocyte binding in vitro.RESULTS: VDR and CYP24A1 were expressed in >2 fold higher fraction of spermatozoa from normal than infertile men (p<0.01). Concentrations of 25-OHD, 24,25(OH)2D, and 1,25(OH)2D3 were undetectable in seminal fluid but high in ovarian follicular fluid. Follicular concentrations of 1,25(OH)2D3 induced a modest increase in [Ca(2+)]i and sperm-oocyte binding in vitro (p<0.05).CONCLUSION: Presence of VDR and CYP24A1 mainly in spermatozoa of higher quality supports that 1,25(OH)2D3 available in the female reproductive tract may promote selection of the best gametes for fertilization.

AB - CONTEXT: The vitamin D receptor (VDR) and enzymes involved in activation (CYP2R1, CYP27B1) and inactivation (CYP24A1) of vitamin D are expressed in ovary, testes, and spermatozoa.OBJECTIVE: Determine responsiveness to 1,25-dihydroxyvitamin D (1,25(OH)2D3) in spermatozoa from normal and infertile men, identify site of exposure and how 1,25(OH)2D3 influences sperm function.DESIGN: Spermatozoa expressing VDR, CYP2R1, CYP27B1, and CYP24A1 were analyzed in normal and infertile men. 25-hydroxyvitamin D (25-OHD), 24,25-dihydroxyvitamin D (24,25(OH)2D3), and 1,25(OH)2D3 were measured in serum, seminal fluid, cervical secretions and ovarian follicular fluid. 1,25(OH)2D3 was tested on human spermatozoa.SETTING: Tertiary centre for fertility.PARTICIPANTS: Protein expression in spermatozoa and semen quality were assessed in 230 infertile and 114 healthy men. Vitamin D metabolites were measured in fluids from 245 men and 13 women, while 74 oocytes and 17 semen donors were used for sperm-function tests.MAIN OUTCOME MEASURES: VDR and CYP24A1 expressions in spermatozoa, fluid concentrations of 25-OHD, 24,25(OH)2D3 and 1,25(OH)2D3, and 1,25(OH)2D3-induced effects on intracellular calcium concentration ([Ca(2+)]i) and sperm-oocyte binding in vitro.RESULTS: VDR and CYP24A1 were expressed in >2 fold higher fraction of spermatozoa from normal than infertile men (p<0.01). Concentrations of 25-OHD, 24,25(OH)2D, and 1,25(OH)2D3 were undetectable in seminal fluid but high in ovarian follicular fluid. Follicular concentrations of 1,25(OH)2D3 induced a modest increase in [Ca(2+)]i and sperm-oocyte binding in vitro (p<0.05).CONCLUSION: Presence of VDR and CYP24A1 mainly in spermatozoa of higher quality supports that 1,25(OH)2D3 available in the female reproductive tract may promote selection of the best gametes for fertilization.

UR - https://www.scopus.com/pages/publications/85015176312

U2 - 10.1210/jc.2016-3008

DO - 10.1210/jc.2016-3008

M3 - Journal article

C2 - 27977320

SN - 0021-972X

VL - 102

SP - 950

EP - 961

JO - The Journal of clinical endocrinology and metabolism

JF - The Journal of clinical endocrinology and metabolism

IS - 3

ER -

Selection of high quality spermatozoa may be promoted by activated vitamin D in the woman

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